Protein Info for BWI76_RS10765 in Klebsiella michiganensis M5al

Annotation: pyrimidine utilization protein D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 92 to 96 (5 residues), see Phobius details amino acids 100 to 100 (1 residues), see Phobius details amino acids 102 to 117 (16 residues), see Phobius details TIGR03611: pyrimidine utilization protein D" amino acids 2 to 258 (257 residues), 345.8 bits, see alignment E=7.4e-108 PF00561: Abhydrolase_1" amino acids 15 to 239 (225 residues), 81.4 bits, see alignment E=1.3e-26 PF12697: Abhydrolase_6" amino acids 17 to 241 (225 residues), 42.6 bits, see alignment E=1.8e-14 PF12146: Hydrolase_4" amino acids 39 to 227 (189 residues), 45.3 bits, see alignment E=1e-15

Best Hits

Swiss-Prot: 88% identical to RUTD_KLEVT: Putative aminoacrylate hydrolase RutD (rutD) from Klebsiella variicola (strain At-22)

KEGG orthology group: K09023, protein RutD (inferred from 88% identity to kva:Kvar_3344)

Predicted SEED Role

"Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1Y8 at UniProt or InterPro

Protein Sequence (267 amino acids)

>BWI76_RS10765 pyrimidine utilization protein D (Klebsiella michiganensis M5al)
MMKLNISPAPYSGAPAVVLSAGLGGAGGYWLPQRAALEPHYQLVSYDHNGTGENGSPLPT
GYSMAAMAQELYAALQAAGIERFALVGHALGALIGLQLALDYPAAVSALVLVNGWLTLSP
HTRRCFLVRERLLHAGGAQAWVEAQPLFLYPAEWMASRLPRLEAEDALAISHFQGKENLL
KRLNALKQADFSRRAAAITCPTLIISAADDLLVPASCSRVLQAAIPGSQLAEMAWGGHAC
NVTDTAAFNPILCDGLAAMLPVPQENL