Protein Info for BWI76_RS10675 in Klebsiella michiganensis M5al

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 833 PF00381: PTS-HPr" amino acids 6 to 85 (80 residues), 59.5 bits, see alignment E=6.8e-20 PF05524: PEP-utilisers_N" amino acids 120 to 237 (118 residues), 44 bits, see alignment E=5.7e-15 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 180 to 677 (498 residues), 477 bits, see alignment E=3.8e-147 PF00391: PEP-utilizers" amino acids 264 to 337 (74 residues), 52.6 bits, see alignment E=7.5e-18 PF02896: PEP-utilizers_C" amino acids 363 to 653 (291 residues), 383.1 bits, see alignment E=2.1e-118 PF00359: PTS_EIIA_2" amino acids 689 to 829 (141 residues), 117.9 bits, see alignment E=9e-38

Best Hits

Swiss-Prot: 80% identical to PTFX2_ECOLI: Multiphosphoryl transfer protein 2 (ptsA) from Escherichia coli (strain K12)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11189, phosphocarrier protein K11201, PTS system, fructose-specific IIA-like component [EC: 2.7.1.69] (inferred from 80% identity to ecm:EcSMS35_4395)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1W5 at UniProt or InterPro

Protein Sequence (833 amino acids)

>BWI76_RS10675 phosphoenolpyruvate--protein phosphotransferase (Klebsiella michiganensis M5al)
MALVIEFICDLPNGVHARPASLVETLCNTFSSSVEWLNIRTEGRGNAKSALAIIGTNTLK
GDRCELIVSGEDEEQAFAALSAFIRDEFPHCDAPLPTAEQMDVQPVPESLSRLNPTLFHA
LPVCAGSAGGRLTHLKSLDLRDLGALPAAGAIEQEQAALDKGLTLLVKNIEFRLLDNDGT
ASAILEAHRSLATDASLRQHLLDGLLSGLSCAEAVVASGEHFCAQFSASGNSYLQERVLD
VRDVCFQLLQHIYGEARFPAPGKLTEEAICLADELTPSQFLELDKTLLKGLLLRSGGTTS
HTVILARSFNIPTLVGVDMEALLPWVDRRVQIDGNAGLVVVNPNQAVARYYQQEAWVQAQ
IRRQQQAWLDKEGRTEDGIRLEVAANIAHSVEATAAFNNGAQSVGLFRTEMLYMDRPSAP
SENELYNIFCQALEPANGRGIIIRTMDIGGDKPVAYLNIPAENNPFLGYRAVRIYAEYQA
LFRTQLRAILRASAHGALKIMIPMISSMEEILWVKEQLADAKQSLRSEHIPFDEKIPLGI
MLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYA
VQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPATKARLAQLDSRACRQL
LNQAMQCRTSLEVEHLLAQFRMTQQDAPLITPQCITLNSDWRSKEEVIKGMTDNLLLAGR
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLAQPVAWGDDEAQFV
IMLTLNKHSAGDQHMRIFSRLARRIMHAEFRQSLVTAQSSEAIAALLQRELEL