Protein Info for BWI76_RS10550 in Klebsiella michiganensis M5al
Annotation: AtzE family amidohydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 84% identity to kpu:KP1_2819)Predicted SEED Role
"Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases"
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B214 at UniProt or InterPro
Protein Sequence (465 amino acids)
>BWI76_RS10550 AtzE family amidohydrolase (Klebsiella michiganensis M5al) MKLPEMTIHDIQRALASGELSAKEIARHTLENIARVNPQINAWTHISEKRMLAEAENIDT LRREKKPLPALAGVPYAVKNLFDVAGHTTLAGAQLLGDRPAAVADSWAVRQLLSAGALLS GMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAAAVAAGLVHFSLGSDTNGSI RVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARCVSDLSAVYDALQGRDPGDGF QADKASERTANLLSRGLEGLRCATLGGYFTTWCDNDARAAVARVAGALAAHEELIFPEAE LARSAAFIISAAEGGNQYLPALRSQAERFEPNSRERLLAGAMIPSAWYLQAQRFRRHAQQ AFKALFAHADVLIAPATPCSATLIGAQNMEINGQPLPIRASMGMLTQPISFLGLPVVTVP LRTASGQPIGLQLIAAPFNEQACLRVARALEETGVTDARPVEIAA