Protein Info for BWI76_RS09925 in Klebsiella michiganensis M5al

Annotation: 23S rRNA (guanine(2445)-N(2))/(guanine(2069)-N(7))- methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 transmembrane" amino acids 458 to 478 (21 residues), see Phobius details PF02926: THUMP" amino acids 21 to 153 (133 residues), 75.6 bits, see alignment E=1.8e-24 PF01170: UPF0020" amino acids 162 to 375 (214 residues), 211.1 bits, see alignment E=5.5e-66 PF10672: Methyltrans_SAM" amino acids 474 to 657 (184 residues), 79.7 bits, see alignment E=8.7e-26 PF03602: Cons_hypoth95" amino acids 531 to 628 (98 residues), 36.6 bits, see alignment E=1.5e-12

Best Hits

Swiss-Prot: 96% identical to RLMKL_KLEP7: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_15305)

MetaCyc: 89% identical to fused 23S rRNA m2G2445 methyltransferase and 23S rRNA m7G2069 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6950 [EC: 2.1.1.264]; RXN-11574 [EC: 2.1.1.264, 2.1.1.173]

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173 or 2.1.1.264

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1M3 at UniProt or InterPro

Protein Sequence (701 amino acids)

>BWI76_RS09925 23S rRNA (guanine(2445)-N(2))/(guanine(2069)-N(7))- methyltransferase (Klebsiella michiganensis M5al)
MNSLFASTARGLEELLKTELEGLGAMDCQLVQGGVHFQGDTRLLYQSLMWSRLASRIMLP
LGQCSVYSDLDLYLGVQAIPWTEIFSQDATFAVHFSGLNEEIRNSQYGALKVKDAIVDSF
TRKNLPRPNVDRENPDLRINVWLNKETAHISLDLSGEGLHLRGYRDGTGMAPIKENLAAA
IVMRSGWATGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGHWGFGGWAQHDDAIWKEVK
AEAQTRARQGLAAYESRFYGSDVDARVIERAQRNARRAGIGELITFEVKDVAQLSNPLPK
GPYGTVISNPPYGERLESEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
KQFKAKNGPLDCVQKNYHLAENEGGKTAMLAEDFANRLRKNLKKYEKWARQEGIECYRLY
DADLPEYNVAIDRYADWVVVQEYAPPKTVDAHKARQRLFDIIAATIAVLGIAPNKLVLKT
RERQKGKNQYQKMGEKGEFIEVQEYNARLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKD
FLNLFSYTGSASVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLMQADVLGW
LRETTEQFDLIFIDPPTFSNSKRMDDAFDVQRDHLRLMTDLKRLLRKGGTIMFSNNKRGF
RMDHDGLAQLGLKAQEITQKTLSQDFARNRQIHNCWLITAA