Protein Info for BWI76_RS09905 in Klebsiella michiganensis M5al

Annotation: FMN reductase (NADPH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03358: FMN_red
  • amino acids 1 to 142 (142 residues), 122.7 bits, E=1e-39
• PF02525: Flavodoxin_2
  • amino acids 1 to 92 (92 residues), 34.3 bits, E=2.1e-12
• TIGR03567: FMN reductase
  • amino acids 2 to 171 (170 residues), 237.4 bits, E=4.4e-75

Best Hits

Swiss-Prot: 79% identical to SSUE_ECOLI: FMN reductase (NADPH) (ssuE) from Escherichia coli (strain K12)

KEGG orthology group: K00299, FMN reductase [EC: 1.5.1.29] (inferred from 94% identity to kpe:KPK_3595)

MetaCyc: 79% identical to NADPH-dependent FMN reductase (Escherichia coli K-12 substr. MG1655)
RXN-12444 [EC: 1.5.1.38]

Predicted SEED Role

"FMN reductase (EC 1.5.1.29)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.5.1.29)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
• two-component alkanesulfonate monooxygenase (2/2 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• bacterial bioluminescence (4/8 steps found)
• dibenzothiophene desulfurization (1/5 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.29

Use Curated BLAST to search for 1.5.1.29 or 1.5.1.38

Sequence Analysis Tools

See A0A285B1W6 at UniProt or InterPro

Protein Sequence (191 amino acids)

>BWI76_RS09905 FMN reductase (NADPH) (Klebsiella michiganensis M5al)
MRVITLAGSPRYPSRSSALLEYARETLSAADIEVCHWHLQNFAPEDLLYARFDNPALHAL
NEQLAGADGLIIATPVYKASFSGALKTLLDLLPERALEGKIVLPLATGGTIAHMLAVDYA
LKPVLNALKAQEILHGVFADDSQVTDYQHKPQFTPNLQGRLDQALETFWQALHRPSSRAP
ALKNLQRVAHV