Protein Info for BWI76_RS09875 in Klebsiella michiganensis M5al

Annotation: phosphoporin PhoE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13609: Porin_4" amino acids 13 to 336 (324 residues), 74.5 bits, see alignment E=1.4e-24 PF00267: Porin_1" amino acids 28 to 359 (332 residues), 421.5 bits, see alignment E=3.1e-130

Best Hits

Swiss-Prot: 66% identical to YEDS_ECOLX: Outer membrane protein YedS (yedS) from Escherichia coli

KEGG orthology group: None (inferred from 93% identity to kpe:KPK_3603)

MetaCyc: 56% identical to outer membrane porin N (Escherichia coli K-12 substr. MG1655)
RXN0-2481

Predicted SEED Role

"Outer membrane protein F precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1L2 at UniProt or InterPro

Protein Sequence (359 amino acids)

>BWI76_RS09875 phosphoporin PhoE (Klebsiella michiganensis M5al)
MMKRKILAAVIPALLVAGAANAAEIYNKNGNKLDFYGKMVGEHVWTTNGDTSSDDTTYAR
IGLKGETQINDQLIGYGQWEYNMDASNVEGKQTTKTRLAFAGLKAGEYGSFDYGRNYGAI
YDVEAATDMLVEWGGDGWSYTDNFMTARTNGVATYRNSDFFGLVDGLSFALQYQGKNKDR
DVKKQNGDGFSTAATYAFDNGVALSAGYANSSRTGDQKADKNGEKAEAWATSAKYDANNV
YAAVMYSQTYNMTPENEGNYFANKTQNFEAVVQYQFDFGLRPSIGYVQTKGKDLRSRAGF
SGGDADLVKYVEVGTWYYFNKNMNVYAAYKFNQLDDNDYTKAAGVATDDQAAVGIVYQF