Protein Info for BWI76_RS09800 in Klebsiella michiganensis M5al
Annotation: tetraacyldisaccharide 4'-kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to LPXK_KLEP7: Tetraacyldisaccharide 4'-kinase (lpxK) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
KEGG orthology group: None (inferred from 90% identity to eae:EAE_15175)MetaCyc: 79% identical to tetraacyldisaccharide 4'-kinase (Escherichia coli K-12 substr. MG1655)
Tetraacyldisaccharide 4'-kinase. [EC: 2.7.1.130]
Predicted SEED Role
"Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 2.7.1.130)
MetaCyc Pathways
- superpathway of (Kdo)2-lipid A biosynthesis (17/17 steps found)
- lipid IVA biosynthesis (P. gingivalis) (9/9 steps found)
- lipid IVA biosynthesis (E. coli) (6/6 steps found)
- lipid IVA biosynthesis (H. pylori) (6/6 steps found)
- lipid IVA biosynthesis (P. putida) (6/6 steps found)
- lipid IVA biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
- lipid IVA biosynthesis (generic) (6/6 steps found)
- lipid IVA biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (5/6 steps found)
- superpathway of Kdo2-lipid A biosynthesis (18/25 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.130
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B1U4 at UniProt or InterPro
Protein Sequence (326 amino acids)
>BWI76_RS09800 tetraacyldisaccharide 4'-kinase (Klebsiella michiganensis M5al) MIARIWSGESPLWRLLLPLSWLYGLVSGLIRLSYKLGWRKAWRAPVPVVVVGNLTAGGNG KTPVVVWLVEQLQQRGIRVGVVSRGYGGKAASYPLLLNPDTRTGEAGDEPVLIYQRTGAP VAVSPVRSDAVQALLAAKDLQLIVTDDGLQHYKLARDKEIVVIDGVRRFGNGWWLPAGPM RERASRLRSVDAVIVNGGLARPGEIPMQLRPGLAVNLRTGERRDVAAFDNVVAMAGIGHP PRFFATLESCGVQPVKTVALADHQALTQSAVAALATARQTLLMTEKDAVKCREFAQANWW YLPVDAIMTDERAQRLLTDLITLAQR