Protein Info for BWI76_RS09760 in Klebsiella michiganensis M5al

Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 PF00275: EPSP_synthase" amino acids 7 to 420 (414 residues), 496 bits, see alignment E=3.9e-153 TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase" amino acids 14 to 423 (410 residues), 475.8 bits, see alignment E=5.5e-147

Best Hits

Swiss-Prot: 94% identical to AROA_KLEP7: 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 93% identity to enc:ECL_02745)

MetaCyc: 89% identical to 3-phosphoshikimate 1-carboxyvinyltransferase (Escherichia coli K-12 substr. MG1655)
3-phosphoshikimate 1-carboxyvinyltransferase. [EC: 2.5.1.19]

Predicted SEED Role

"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1M1 at UniProt or InterPro

Protein Sequence (427 amino acids)

>BWI76_RS09760 3-phosphoshikimate 1-carboxyvinyltransferase (Klebsiella michiganensis M5al)
MESLTLQPIARVDGTINLPGSKSVSNRALLLAALARGTTVLTNLLDSDDVRHMLNALSAL
GVHYTLSSDRTRCEVTGNGGPLQAAEALELYLGNAGTAMRPLAAALCLGRNDIVLTGEPR
MKERPIGHLVDALRQGGAQIDYLEQENYPPLRLRGGFSGGQVEVDGSVSSQFLTALLMAA
PLAPQDTAIVIKGDLVSKPYIDITLHLMKTFGVEVENQSYQRFIVRGNQHYLAPGDYLVE
GDASSASYFLAAGAIKGGTVKVTGIGRNSVQGDIRFADVLEKMGATVTWGDDFIACTHGE
LNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNIYNWRVKETDRLFAMATELRKVGAEVE
EGEDYIRITPPAKLQFAEIGTWNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQL
ARISTPA