Protein Info for BWI76_RS09735 in Klebsiella michiganensis M5al

Annotation: formate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 11 to 760 (750 residues), 1430.2 bits, E=0
• PF02901: PFL-like
  • amino acids 23 to 614 (592 residues), 717.9 bits, E=1.4e-219
• PF01228: Gly_radical
  • amino acids 631 to 741 (111 residues), 123.6 bits, E=5.1e-40

Best Hits

Swiss-Prot: 94% identical to PFLB_ECOLI: Formate acetyltransferase 1 (pflB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to eae:EAE_15120)

MetaCyc: 94% identical to pyruvate formate-lyase (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• superpathway of N-acetylneuraminate degradation (22/22 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
• superpathway of L-threonine metabolism (18/18 steps found)
• mixed acid fermentation (16/16 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Sequence Analysis Tools

See A0A285B1E7 at UniProt or InterPro

Protein Sequence (760 amino acids)

>BWI76_RS09735 formate acetyltransferase (Klebsiella michiganensis M5al)
MSELNEKLATAWEGFAKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATDATTKLWDTVME
GVKQENRTHAPVDFDTSLASTITSHDAGYIEKGLEKIVGLQTEAPLKRAIIPFGGIKMVE
GSCKAYDRELDPMLKKIFTEYRKTHNQGVFDVYTKDILNCRKSGVLTGLPDAYGRGRIIG
DYRRVALYGIDFLMKDKYAQFVSLQEKLENGEDLEATIRLREEISEQHRALGQIKEMAAK
YGCDISGPATTAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSSFLDIFIERDLKAGKITEQ
DAQEMIDHLVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGVDGRTLVTKNSFRFLNT
LYTMGPSPEPNITILWSEKLPLSFKKYAAKVSIDTSSLQYENDDLMRPDFNNDDYAIACC
VSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNFDEVMERM
DHFMDWLAKQYVTALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAI
KYAKVKPIRDENGLAVDFEIEGEYPQFGNNDSRVDDMAVDLVERFMKKIQKLHTYRNAIP
TQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYA
KDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAM
ENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQTM