Protein Info for BWI76_RS09555 in Klebsiella michiganensis M5al

Annotation: pyruvate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 4 to 116 (113 residues), 109.1 bits, E=1.8e-35
• PF00205: TPP_enzyme_M
  • amino acids 191 to 319 (129 residues), 116 bits, E=1.6e-37
• PF02775: TPP_enzyme_C
  • amino acids 379 to 525 (147 residues), 147.6 bits, E=3.7e-47

Best Hits

Swiss-Prot: 93% identical to POXB_ECOLI: Pyruvate dehydrogenase [ubiquinone] (poxB) from Escherichia coli (strain K12)

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 96% identity to kpn:KPN_00904)

MetaCyc: 93% identical to pyruvate oxidase (Escherichia coli K-12 substr. MG1655)
RXN-11496 [EC: 1.2.5.1]; RXN0-2022 [EC: 1.2.5.1]

Predicted SEED Role

"Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.2.2)

MetaCyc Pathways

• pyruvate to cytochrome bd oxidase electron transfer (2/2 steps found)
• pyruvate fermentation to acetoin (2/3 steps found)
• pyruvate to cytochrome bo oxidase electron transfer (1/2 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.2.2 or 1.2.5.1

Sequence Analysis Tools

See A0A285B1I0 at UniProt or InterPro

Protein Sequence (572 amino acids)

>BWI76_RS09555 pyruvate oxidase (Klebsiella michiganensis M5al)
MKQTVAAYIAKTLEQAGVKRIWGVTGDSLNGLSDSLNRMGTIDWMPTRHEEVAAFAAGAE
AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
FRECSHYCELVSSPEQIPQVLAIAMRKAVINRGVSVVVLPGDVALKPAPESASSHWYHAP
LPQVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHKELVAFAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPGSIGA
HSKVDMALVGDIKSTLNALLPLLEEKTDRKFLDKALEHYRDARKGLDDLAKPSDKTLHPQ
YLAQRISHFADEDAIFTCDVGTPTVWAARYLQMNGKRRLLGSFNHGSMANAMPQAIGAKA
TAPDRQVVAMCGDGGFSMLMGDFLSLAQMKLPVKIVIFNNSVLGFVAMEMKAGGYLTDGT
ELHATNFARIAEACGIKGIRVERASEVDEALQTAFATDGPVLVDVVVASEELAMPPQIKL
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR