Protein Info for BWI76_RS09245 in Klebsiella michiganensis M5al

Annotation: bacteriophage replication gene A protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 803 transmembrane" amino acids 268 to 284 (17 residues), see Phobius details PF05840: Phage_GPA" amino acids 198 to 519 (322 residues), 422.2 bits, see alignment E=6.1e-131

Best Hits

Swiss-Prot: 88% identical to ENDPH_SALPA: Probable replication endonuclease from prophage-like region (SPA2590) from Salmonella paratyphi A (strain ATCC 9150 / SARB42)

KEGG orthology group: None (inferred from 96% identity to ent:Ent638_1350)

Predicted SEED Role

"Phage replication protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (803 amino acids)

>BWI76_RS09245 bacteriophage replication gene A protein (Klebsiella michiganensis M5al)
MTDISSGRPVAPHVSEAPGSEGNATGAYPWTSPKKAVNPYLDPADVAPVSALSNLITLYA
ADNEQEQLRREALSDEVWERYFFNESRDPVQREMEQDQLISRARMAHEQQRFNPDLVILA
NVSAEPAHVSKPLLERIKFFQGLGRPKAYSRYLRETIMPCLERLECVRESQVSASFRFMA
SHEGLEGLLVLPEMNQEQVKRLSTLVAAHMSLCLDAACSDLFVTDDVKPEHIRQSWEKVA
AEAMRLDVIPPAFEQLRRKKRRRKPVPYDLIPGSLARMLCADWWYRKLWQMRCEWREEQL
RAVCLVNRKASPYVSYEAVIHKREQRRKSLEFFQSHELVNADGDTLDMEDVVNASSSNPA
HRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHATLNNGRPNPKWTSETVRQSSDYL
VDTFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRTLTALLRKFAIRED
RAELGNNTGPRFKSELINPRKGTPTSYIAKYISKNIDGRGLAKEISKETGKSLRDSAEHV
SAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAARAQGDKKAGVPVLENPRLDAVLAAAD
VGCFATYIMKQGGVLVPRKNHLIRTAYELNDEPGTYGDHGIRIYGIWSPIVEGRICTHAM
KWKMVRKAVDVQEATADQGACAPWTRGNNCPPVEKMYQAGGELPGSEEPAALPDFENMSK
KELRELTARLRLVKPKRRKGYKQEITEHQRLQLDAELRSRGFDASETEVDLLLRGGSLPS
GAGLRLFYRNQRLQEDDKWRQWY