Protein Info for BWI76_RS09070 in Klebsiella michiganensis M5al

Annotation: formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01774: glycyl radical enzyme, PFL2/glycerol dehydratase family
  • amino acids 9 to 810 (802 residues), 1350.8 bits, E=0
• PF02901: PFL-like
  • amino acids 11 to 671 (661 residues), 697.9 bits, E=1.6e-213
• PF01228: Gly_radical
  • amino acids 688 to 792 (105 residues), 110.6 bits, E=5.5e-36

Best Hits

Swiss-Prot: 93% identical to PFLF_ECOLI: Putative formate acetyltransferase 3 (ybiW) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_14720)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• superpathway of N-acetylneuraminate degradation (22/22 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
• superpathway of L-threonine metabolism (18/18 steps found)
• mixed acid fermentation (16/16 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Sequence Analysis Tools

See A0A285B1A4 at UniProt or InterPro

Protein Sequence (810 amino acids)

>BWI76_RS09070 formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme (Klebsiella michiganensis M5al)
MTTLKLDTLSPRIQAHKMALVHIVKPPVCTERAQHYTEAYQQHLDKPIPVRRALALAHHL
AERTIWIKHDELIVGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVL
HEVCPWWRGQTVQDRCYGMFTDEQKALLATGIIKAEGNMTSGDAHLAVNYPLLLEKGLDG
MRAKVAERRSRINLTVLEDLHGEQFLKAIDIVLEAVSEHCKRYAELARSMAATESREARR
DELLAIAENCDIIAHEPPKTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
VELAQSLDREHAIELLHCCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGNAQ
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLATLEGGRDATSGQ
VFLPQEHALSKGNFDNFEQVLADWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFDQGVIGQQALAKALAEDFE
GLTHEQLRQRLINGAPKYGNDEDSVDMLLARAYQTYIDELKLYHNPRYGRGPIGGNYYAG
TSSISANVPFGAQTMATPDGRKAHSPLAEGASPASGTDHLGPTAVISSVGKLPTGSILGG
VLLNQKLNPATLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDL
VVRVAGYSAFFTALSPDAQDDIIARTEHTL