Protein Info for BWI76_RS08715 in Klebsiella michiganensis M5al

Annotation: acyl-CoA thioesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01095: Pectinesterase
  • amino acids 194 to 341 (148 residues), 38.5 bits, E=3e-14

Best Hits

Swiss-Prot: 80% identical to YBHC_ECOLI: Putative acyl-CoA thioester hydrolase YbhC (ybhC) from Escherichia coli (strain K12)

KEGG orthology group: K01051, pectinesterase [EC: 3.1.1.11] (inferred from 90% identity to kpu:KP1_1741)

Predicted SEED Role

"Pectinesterase (EC 3.1.1.11)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.1.1.11)

MetaCyc Pathways

• pectin degradation I (3/3 steps found)
• pectin degradation II (3/5 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.11

Use Curated BLAST to search for 3.1.1.11

Sequence Analysis Tools

See A0A285B161 at UniProt or InterPro

Protein Sequence (427 amino acids)

>BWI76_RS08715 acyl-CoA thioesterase (Klebsiella michiganensis M5al)
MNTYSVSRLALALAFGVTLSACSSTPADQQPSTQTAPGTASRPVLTADEAKNFLPASYFQ
SLDPNAAAWTPSAISLPAQPDFVVGPAGTQGVTHTTIQAAVDAAITRHSNRRQFIAIMPG
EYAGTVYVPAAPGALTLYGTGEKPIDVKISAAIDSEMDPVSWRHQVNPGGKYMPGKPAWY
MYDSCQNKRTATIGVMCSAVFWSQNNDLQLQNLTIANNLGDSVDAGTHQAVALRSDGDRV
QINNVNILGRQNTFFVTNSGVQNRLQDNRQTRTLVSNSYIEGDVDIVSGRGAVVFDNTDF
RVVNSRTQKEGYVFAPATLKSVTYGFLAVNSRFTASGDNVAQLGRSLDVDGNTNGQVVIR
DSVINEGFNVAKPWADAVASNRPFSGNTGTADAKGNPQRNLNDANFNRMWEYNNRGVGSV
VVAEPKQ