Protein Info for BWI76_RS08645 in Klebsiella michiganensis M5al

Annotation: molybdate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF13531: SBP_bac_11" amino acids 29 to 254 (226 residues), 201.1 bits, see alignment E=2.3e-63 PF01547: SBP_bac_1" amino acids 34 to 245 (212 residues), 84.2 bits, see alignment E=1.7e-27 TIGR01256: molybdate ABC transporter, periplasmic molybdate-binding protein" amino acids 34 to 253 (220 residues), 280.6 bits, see alignment E=5.4e-88

Best Hits

Swiss-Prot: 84% identical to MODA_ECOLI: Molybdate-binding protein ModA (modA) from Escherichia coli (strain K12)

KEGG orthology group: K02020, molybdate transport system substrate-binding protein (inferred from 89% identity to esc:Entcl_3066)

MetaCyc: 84% identical to molybdate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]

Predicted SEED Role

"Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)" in subsystem Molybdenum cofactor biosynthesis or Transport of Molybdenum (TC 3.A.1.8.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0Y6 at UniProt or InterPro

Protein Sequence (257 amino acids)

>BWI76_RS08645 molybdate ABC transporter substrate-binding protein (Klebsiella michiganensis M5al)
MTGQWLRFIAGATLTFSVAGHALAEEGKVTVFAAASLTNAMQDIAKEYKKEKNVDVVSSF
ASSSTLARQIEAGAPADLFISADQKWMDYAVEKKSIDTASRATLLGNSLVVVAPKASAQG
DISINDKTDWTSLLKGGRLAVGDPEHVPAGIYAKEALQKLGAWETLSPKLAPAEDVRGAL
ALVERSEAPLGIVYGSDAVASKGVKVVGTFPEDSHKKVEYPLAIVDGHKNATVSAFYDYL
KGPEASAIFKRYGFSTH