Protein Info for BWI76_RS08475 in Klebsiella michiganensis M5al
Annotation: methylaspartate ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to MAAL_CITAM: Methylaspartate ammonia-lyase from Citrobacter amalonaticus
KEGG orthology group: K04835, methylaspartate ammonia-lyase [EC: 4.3.1.2] (inferred from 96% identity to cko:CKO_02419)MetaCyc: 58% identical to methylaspartase subunit (Clostridium tetanomorphum)
Methylaspartate ammonia-lyase. [EC: 4.3.1.2]
Predicted SEED Role
"Methylaspartate ammonia-lyase (EC 4.3.1.2)" (EC 4.3.1.2)
MetaCyc Pathways
- L-glutamate degradation VI (to pyruvate) (2/4 steps found)
- L-glutamate degradation VIII (to propanoate) (7/11 steps found)
- L-glutamate degradation VII (to butanoate) (7/12 steps found)
- methylaspartate cycle (12/19 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B109 at UniProt or InterPro
Protein Sequence (413 amino acids)
>BWI76_RS08475 methylaspartate ammonia-lyase (Klebsiella michiganensis M5al) MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFIYTGAPVTPGFTSVRQAGECVSVQLILE NGAVAVGDCAAVQYSGAGGRDPLFLAENFIPFLNDHIKPLLEGRDVDTFLPNARFFDKLR IDGHLLHTAVRYGLSQALLDATALASGRLKAEVVCDEWQLPCIPEAIPLFGQSGDDRYIA VDKMILKGVDVLPHALINNVEEKLGLKGEKLREYVRWLSDRILSLRASPRYRPTLHIDVY GTIGLIFDMDPVRCADYIASLEKEAQGLPLYIEGPVDAGNKPDQIRMLTAITKELTRLGS GVKIVADEWCNTYQDIIDFTDAGSCHMVQIKTPDLGGIHNIVDAVLYCNKHAMEAYQGGT CNETEISARTCVHVALAARPMRMLIKPGMGFDEGLNIVFNEMNRTIALLQAKD