Protein Info for BWI76_RS08415 in Klebsiella michiganensis M5al
Annotation: 2-oxoglutarate dehydrogenase subunit E1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to ODO1_ECO57: 2-oxoglutarate dehydrogenase E1 component (sucA) from Escherichia coli O157:H7
KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 95% identity to cko:CKO_02434)MetaCyc: 93% identical to subunit of E1(0) component of 2-oxoglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Oxoglutarate dehydrogenase (succinyl-transferring). [EC: 1.2.1.105, 1.2.4.2]
Predicted SEED Role
"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
- superpathway of glyoxylate bypass and TCA (11/12 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- TCA cycle I (prokaryotic) (9/10 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
- TCA cycle III (animals) (8/10 steps found)
- TCA cycle VIII (Chlamydia) (5/6 steps found)
- TCA cycle II (plants and fungi) (7/9 steps found)
- 2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA (1/5 steps found)
- L-tryptophan degradation III (eukaryotic) (4/15 steps found)
- L-tryptophan degradation XI (mammalian, via kynurenine) (4/23 steps found)
- superpathway of L-lysine degradation (12/43 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Lysine degradation
- Tryptophan metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.105 or 1.2.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B164 at UniProt or InterPro
Protein Sequence (935 amino acids)
>BWI76_RS08415 2-oxoglutarate dehydrogenase subunit E1 (Klebsiella michiganensis M5al) MQNGAMKAWLDSSYLSGSNQSWIEQLYEDFLTDPDSVDANWRSMFQQLPGTGVKPDQFHS KTRDYFRRLAKDASRYTSSISDPDTNVKQVKVLQLINAYRFRGHQHANLDPLGLWQQERV ADLDPAYHDLTEADFQEIYNVGSFAIGKDTMKLGDLISALKQTYCGSIGAEYMHITSTEE KRWIQQRIESVAGKASFSAEEKKRFLSELTAAEGLERYLGAKFPGAKRFSLEGGDALIPM LKEIIRHAGKSGTREVVLGMAHRGRLNVLVNVLGKKPQDLFDEFAGKHKEHLGTGDVKYH MGFSSDMETEGGLVHLALAFNPSHLEIVSPVVIGSVRARLDRLDEPSSNKVLPITIHGDA AVTGQGVVQETLNMSKARGYEVGGTVRIVINNQVGFTTSNPLDARSTPYCTDIGKMVQSP IFHVNADDPEAVAFVTRLALDFRNTFKRDVFIDLVCYRRHGHNEADEPSATQPLMYQKIK KHPTPRKIYADKLEADKVATLEDATEQVNLYRDALDAGECVVQEWRPMNMHSFTWSPYLN HEWDESYPDKVEPKRLQELAKRISTVPEAVEMQSRVAKIYGDRQAMAAGEKLFDWGGAEN LAYATLVDEGISVRLSGEDSGRGTFFHRHAVIHNQKNGSTYTPLQHVHNGQGHFKVWDSV LSEEAVLAFEYGYATAEPRTLTIWEAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLL PHGYEGQGPEHSSARLERYLQLCAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLIVMSP KSLLRHPLAVSSMDELANGTFQPAIGEIDDLDPKAVKRVVLCSGKVYYDLLEQRRKKEQK DVAIVRIEQLYPFPHQAVQEALKPYAHVHDFVWCQEEPLNQGAWYCSQHHFREVIPFGAS LRYAGRPASASPAVGYLSVHQKQQQDLVNDALNVD