Protein Info for BWI76_RS08315 in Klebsiella michiganensis M5al

Annotation: K+-transporting ATPase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 682 transmembrane" amino acids 34 to 55 (22 residues), see Phobius details amino acids 61 to 82 (22 residues), see Phobius details amino acids 219 to 242 (24 residues), see Phobius details amino acids 252 to 276 (25 residues), see Phobius details amino acids 584 to 604 (21 residues), see Phobius details amino acids 615 to 635 (21 residues), see Phobius details amino acids 655 to 678 (24 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 2 to 677 (676 residues), 1223.2 bits, see alignment E=0 TIGR01494: HAD ATPase, P-type, family IC" amino acids 71 to 338 (268 residues), 127.8 bits, see alignment E=4e-41 amino acids 372 to 604 (233 residues), 165.5 bits, see alignment E=1.5e-52 PF00122: E1-E2_ATPase" amino acids 114 to 282 (169 residues), 111.8 bits, see alignment E=4.3e-36 PF00702: Hydrolase" amino acids 301 to 530 (230 residues), 96 bits, see alignment E=6.1e-31

Best Hits

Swiss-Prot: 96% identical to KDPB_KLEP3: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_14080)

MetaCyc: 90% identical to K+ transporting P-type ATPase subunit KdpB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-2 [EC: 7.2.2.6]

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12 or 7.2.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B146 at UniProt or InterPro

Protein Sequence (682 amino acids)

>BWI76_RS08315 K+-transporting ATPase subunit B (Klebsiella michiganensis M5al)
MSRKSLALLEPTLVRQALLDAVKKLSPAVQWRNPVMFIVWIGSLLTTLLAIAMASGHIAG
SAGFTAAISLWLWFTVLFANFAEAMAEGRSKAQANSLKGVKKTAFARKLRAPQHDAQIDH
VPAEDLRKGDVVLVEAGDIIPCDGEVIEGGASVDESAITGESAPVIRESGGDFASVTGGT
RILSDWLVIRCSVNPGETFLDRMIAMVEGAQRRKTPNEIALTILLIALTLVFLLATATIW
PFSAWSGNAVSVTVLVALLVCLIPTTIGGLLSAIGVAGMSRMLGANVIATSGRAVEAAGD
VDVLLLDKTGTITLGNRQASAFLPAQGVEEKTLADAAQLSSLADETPEGRSIVVLAKQRF
NLRERDLQSLHATFVPFTAQTRMSGINIDQRMIRKGSVDAIRRHVEANGGHFPADVDKQV
EDVARLGATPLVVAEGERVLGIISLKDIVKGGIKERFAQLRKMGIKTVMITGDNRLTAAA
IAAEAGVDDFLAEATPEAKLALIRQYQSEGRLVAMTGDGTNDAPALAQADVAVAMNSGTQ
AAKEAGNMVDLDSNPTKLIEVVHIGKQMLMTRGSLTTFSIANDVAKYFAIIPAAFAAVYP
PLAMLNVMHLHSPSSAILSAVIFNALIIIFLIPLALKGVSYKPLTASAMLRRNLWVYGLG
GLLVPFIGIKAIDLLLTLTGLM