Protein Info for BWI76_RS08305 in Klebsiella michiganensis M5al

Annotation: two-component sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 895 transmembrane" amino acids 399 to 419 (21 residues), see Phobius details amino acids 425 to 443 (19 residues), see Phobius details amino acids 449 to 466 (18 residues), see Phobius details amino acids 477 to 498 (22 residues), see Phobius details PF02702: KdpD" amino acids 21 to 230 (210 residues), 319.8 bits, see alignment E=2.2e-99 PF13493: DUF4118" amino acids 403 to 508 (106 residues), 100.2 bits, see alignment E=1.7e-32 PF13492: GAF_3" amino acids 530 to 644 (115 residues), 35.9 bits, see alignment E=2.8e-12 PF00512: HisKA" amino acids 664 to 730 (67 residues), 48 bits, see alignment 3.1e-16 PF02518: HATPase_c" amino acids 777 to 883 (107 residues), 96.6 bits, see alignment E=3.7e-31

Best Hits

Swiss-Prot: 83% identical to KDPD_ECOLI: Sensor protein KdpD (kdpD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 91% identity to eae:EAE_14070)

MetaCyc: 83% identical to sensor histidine kinase KdpD (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0S2 at UniProt or InterPro

Protein Sequence (895 amino acids)

>BWI76_RS08305 two-component sensor histidine kinase (Klebsiella michiganensis M5al)
MSDEPLRPDPDRLLQHTAAPHRGKLKVFFGACAGVGKTWAMLAEAQRLRAQGLDILIGVA
ETHGRKETAAMLEGLCTLPQRRLSHRGRYVKEFDLDGALARRPALILVDELAHSNAPGSR
HPKRWQDVEELLEAGIDVFTTVNVQHLESLNDVVSGITGVQVRETVPDPFFDAADDVVLV
DLPPDDLRQRLNEGKVYIGGQAERAIENFFRKGNLIALRELALRRTADRVDEQMRAWRDR
QGQEKVWHTRDAILLCIGHNTGSEKLVRAAARLAARLGSVWHAVYVETPSLHRLPEAKRR
AILAALRLAQELGAETATLADPSEERAVLHYAREHNLGKIVIGRQQKRRWWDRSGFADRL
ARHAPDLDLVVIALDEKAAPLPARASDGRTAMEKWRLQLQGCVVAIALCAVITVVAMQWL
MAFEAANLVMLYLLGVVIIALLYGRWPSVLATVINVVSFDLFFVAPRGTLAVSDVQYLLT
FSVMLTVGLLIGNLTAGVRYQARVARYREQRTRHLYEMSKALAVGRSREDVAATSERFIA
STFQARSQLLLPDETNRLSPLTPQTAAITWDDAIARWSFDKGQPAGAGTDTLPGVPYQIL
PLKSAARTWGLLVVEPANLRQLMIPEQQRLLETFTLLVASALERLTLTASEEQARLNSER
ESLRNSLLAALSHDLRTPLTVLFGQAEILTLDLASEGSKHAPQANEIRQHVLNTTRLVNN
LLDMARIQSGGFNLHKEWLTLEEVVGSALRMLEPGLGGRHIQLALPDPLLLVHVDGPLFE
RVLINLLENAVKYAGTEAHIGITAHVDPHNLLLEVWDNGPGIPAGQEQAIFDKFARGNKE
SAIPGVGLGLAICQAIVEVHGGTISAATRPQGGACFRVTLPREAPPELEDLAEEM