Protein Info for BWI76_RS08225 in Klebsiella michiganensis M5al

Annotation: glutamine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 TIGR00440: glutamine--tRNA ligase" amino acids 28 to 552 (525 residues), 1036.5 bits, see alignment E=0 PF00749: tRNA-synt_1c" amino acids 28 to 337 (310 residues), 431.1 bits, see alignment E=3.2e-133 PF03950: tRNA-synt_1c_C" amino acids 340 to 440 (101 residues), 117.9 bits, see alignment E=2.9e-38 PF20974: tRNA-synt_1c_C2" amino acids 457 to 529 (73 residues), 90 bits, see alignment E=1.9e-29

Best Hits

Swiss-Prot: 97% identical to SYQ_KLEP7: Glutamine--tRNA ligase (glnS) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_14000)

MetaCyc: 93% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0W3 at UniProt or InterPro

Protein Sequence (555 amino acids)

>BWI76_RS08225 glutamine--tRNA ligase (Klebsiella michiganensis M5al)
MSEAEARPTNFIRQIIDEDLATGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
QCNLRFDDTNPVKEDIEYVESIKNDVQWLGFHWSGDVCYSSDYFDQLHQYAVELINKGLA
YVDELSAEEIREYRGTLKEPGKNSPYRDRSVEENLALFEKMRSGGFAEGKACLRAKIDMA
SPFIVMRDPVLYRIKFADHHQTGSKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNISIPVHPRQYEFSRLNLEYTVMSKRKLNQLVTEKHVEGWDDPRMPTISGLRRR
GYTAESIREFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYPQG
ESEMVAMPNHPNKPEMGSREVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIK
AERVEKDAEGNITTIFCTYDADTLSKDPADGRKVKGVIHWVSAAHALPVEIRLYDRLFSV
PNPGAAEDFLAVMNPESLVIKQGFAEPSLAQAQAEKAYQFEREGYFCLDSRYATASHLVF
NRTVGLRDTWTKVGA