Protein Info for BWI76_RS08200 in Klebsiella michiganensis M5al

Annotation: UMP phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 4 to 214 (211 residues), 61.5 bits, E=2.9e-20
• PF13344: Hydrolase_6
  • amino acids 6 to 105 (100 residues), 84.4 bits, E=1e-27
• TIGR01460: HAD hydrolase, family IIA
  • amino acids 6 to 223 (218 residues), 211.4 bits, E=1.9e-66
• PF13419: HAD_2
  • amino acids 163 to 220 (58 residues), 33.8 bits, E=7.6e-12
• PF13242: Hydrolase_like
  • amino acids 174 to 246 (73 residues), 77.4 bits, E=1.3e-25

Best Hits

Swiss-Prot: 92% identical to NAGD_ECOLI: Ribonucleotide monophosphatase NagD (nagD) from Escherichia coli (strain K12)

KEGG orthology group: K02566, NagD protein (inferred from 98% identity to kva:Kvar_3677)

MetaCyc: 92% identical to UMP phosphatase (Escherichia coli K-12 substr. MG1655)
5'-nucleotidase. [EC: 3.1.3.5]

Predicted SEED Role

"Phosphatase NagD predicted to act in N-acetylglucosamine utilization subsystem" in subsystem Chitin and N-acetylglucosamine utilization

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purines degradation in plants (15/18 steps found)
• ureide biosynthesis (7/7 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• NAD salvage (plants) (5/11 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.5

Use Curated BLAST to search for 3.1.3.5

Sequence Analysis Tools

See A0A285B0P4 at UniProt or InterPro

Protein Sequence (250 amino acids)

>BWI76_RS08200 UMP phosphatase (Klebsiella michiganensis M5al)
MTIQNVICDIDGVLMHDNVAVPGAAEFIKRILEKEMPLVMLTNYPSQTGQDLANRFATAG
IDVPDTAFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETR
SFNWEMMHKAAFFVANGARFIATNPDTHGRGYYPACGALCAGIEKISGRKPFYVGKPSPW
IIRSALNKMQAHSEQTVIVGDNLRTDILAGFQAGLETILVLSGVATLDDIDSMPFRPSWI
YPSVAEIDIF