Protein Info for BWI76_RS08195 in Klebsiella michiganensis M5al

Annotation: alpha-glucuronidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 PF07488: Glyco_hydro_67M" amino acids 122 to 440 (319 residues), 476.4 bits, see alignment E=4.3e-147 PF07477: Glyco_hydro_67C" amino acids 441 to 663 (223 residues), 299.2 bits, see alignment E=1.9e-93

Best Hits

KEGG orthology group: K01235, alpha-glucuronidase [EC: 3.2.1.139] (inferred from 59% identity to cle:Clole_0558)

Predicted SEED Role

"Alpha-glucosidase (EC 3.2.1.20)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.139 or 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (667 amino acids)

>BWI76_RS08195 alpha-glucuronidase (Klebsiella michiganensis M5al)
MQPIINDNLPRNYQKCWLPSSTDIPWLIRYLDNVYLPEGEDIIKSTLINEITALNHNIKN
SKSLQEASLSFVLDENNMVSEQYSIDYQNDTIRIVSGGWQGFLYAWFDLLRKASSVLNSP
WQSTQKPAFPVRMINHWDNLDGTVERGYAGNSLFFHNNQIHYDEIRLHHYARLLASVGIN
AIAINNVNVHHHETALIDDSLLPSVAQIAAFFRQYAIRLFLSVNFAAPIETGELSVADPL
DPQVIDWWQTRAEKIYQQIADFGGYIVKADSEHRPGPFTYQRTHADGANMLARTLAPYDG
LLYWRCFVYNCQQQWYDRTTDRAKAAWDNFAPLDGQFLDNVILQIKNGPMDFQVREPVSP
LLGSMPNTNKVMELQITQEYTGQQIDLCWLVPQWKTILNFDTRPGERDATILDILAGHSQ
HHMQHSGITAVVNTGDSVFWTGHPLAQANLYGYGRLLWDPLIDEKSLAEEWCKLSLSTAP
KAVETIRQMLLSSWLTYENYTSPLGVGWMVQPHHHYGPSIDGYEYDSWGTYHYADRYGLG
VDRTSDSGTGYVKQYHPLNCQMYNNPGRCPQELLLFFHHLQYRHVLPSGKTLIQHIYDSH
FSGVNQVELYIQQWKTLEGDITEDIYSEVLQRLVKQLANAQAWRDQINTYFYRKSGIKDS
RNRNIYP