Protein Info for BWI76_RS07915 in Klebsiella michiganensis M5al

Annotation: acireductone synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 2 to 196 (195 residues), 62.2 bits, E=4.5e-21
• TIGR01691: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
  • amino acids 2 to 222 (221 residues), 353.9 bits, E=3.2e-110
• TIGR01549: HAD hydrolase, family IA, variant 1
  • amino acids 116 to 196 (81 residues), 27.2 bits, E=4.7e-10

Best Hits

Swiss-Prot: 100% identical to MTNC_KLEOX: Enolase-phosphatase E1 (mtnC) from Klebsiella oxytoca

KEGG orthology group: K09880, enolase-phosphatase E1 [EC: 3.1.3.77] (inferred from 90% identity to kpe:KPK_3930)

MetaCyc: 100% identical to acireductone synthase [multifunctional] (Klebsiella oxytoca)
Acireductone synthase. [EC: 3.1.3.77, 5.3.2.5]; R83-RXN [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]; 3.1.3.77 [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]

Predicted SEED Role

"2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase (EC 3.1.3.77)" in subsystem Methionine Salvage (EC 3.1.3.77)

MetaCyc Pathways

• L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
• L-methionine salvage cycle III (10/11 steps found)
• L-methionine salvage cycle II (plants) (9/11 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (6/7 steps found)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.77 or 3.1.3.87 or 5.3.2.5

Sequence Analysis Tools

See A0A285B0P5 at UniProt or InterPro

Protein Sequence (229 amino acids)

>BWI76_RS07915 acireductone synthase (Klebsiella michiganensis M5al)
MIRAIVTDIEGTTSDIRFVHNVLFPYARERLAGFVTAQQFVEPVKTILDNLREEIAQPAA
GAEELIATLFAFMDEDRKSTALKALQGIIWRDGYVHGDFTGHLYPDVLPALEKWKSQGID
LYVYSSGSVAAQKLLFGYSDEGDITHLFNGYFDTLVGAKREAQSYRNIAEQLGQPPAAIL
FLSDIHQELDAAEEAGFRTLQLVRGDRDPASHHPQVQRFDDIHPEQIPA