Protein Info for BWI76_RS07715 in Klebsiella michiganensis M5al

Annotation: non-ribosomal peptide synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1295 PF00668: Condensation" amino acids 3 to 442 (440 residues), 284.5 bits, see alignment E=3.8e-88 PF00501: AMP-binding" amino acids 462 to 813 (352 residues), 294.7 bits, see alignment E=2.4e-91 TIGR01733: amino acid adenylation domain" amino acids 482 to 886 (405 residues), 425.6 bits, see alignment E=9.2e-132 PF13193: AMP-binding_C" amino acids 883 to 951 (69 residues), 25.5 bits, see alignment 5.1e-09 PF00550: PP-binding" amino acids 979 to 1041 (63 residues), 45.9 bits, see alignment 1.3e-15 PF00975: Thioesterase" amino acids 1068 to 1284 (217 residues), 181.5 bits, see alignment E=7.2e-57

Best Hits

Swiss-Prot: 78% identical to ENTF_ECOLI: Enterobactin synthase component F (entF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 88% identity to eae:EAE_13615)

MetaCyc: 78% identical to apo-serine activating enzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Enterobactin synthetase component F, serine activating enzyme (EC 2.7.7.-)" in subsystem Siderophore Enterobactin (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0F8 at UniProt or InterPro

Protein Sequence (1295 amino acids)

>BWI76_RS07715 non-ribosomal peptide synthetase (Klebsiella michiganensis M5al)
MTTRLPLVAAQPGIWMAERLSTLPGAWSVAHYVELRGDIDPVLLGRAIITGLSQADTLSM
RFCEDSGEAWQWVDERRVFAEPDYQDLRAASDPHAAALARMQADLGQNLRVDGGNPLVCH
QLLRVGDNRWYWYQRYHHLLVDGFSFPAITRQIAAIYRAWQRGEETPASPFTPFAEVVEE
YQRYYGSDAWQRDKAFWQAQRQALPSPASLSDAPLAGRATSSDIWRMKLEVDAGVFSRLA
ASAPQCQRADLALALTALWLGRLCSRTEYAAGFIFMRRMGSAALTATGPVLNVLPLAVTI
DAGETLAELAARLAGQLKKMRRHQRYDAEQIVRDSGKAAGDEPLFGPVLNIKVFDYQLDI
DGVQAITHTLATGPVNDLELALFPDEAGGLSLEVLANKQRYDKQTLGNHIARLSALLAQF
ADNPALRCGDVNMLSAAEAEQLQRVNATAVDLPLTTLSARVAEQAAKTPDAPALADGRWQ
FSYREMRQQVVALAEQLRARGVKPGDSVAVALPRSVFLTLALHGIVEAGAAWLPLDTGYP
DDRLRMMLEDARPSLLITSEDQRARFSDIPGLESLCYQHPLAAADAAPLALSQPEHTAYI
IFTSGSTGRPKGVMVGQTAIVNRLLWMQDHYPLTADDVVAQKTPCSFDVSVWEFWWPFIA
GAQLVMAEPEAHRDPLAMQQFFARYGVTTTHFVPSMLAAFVASLNTENVASCHTLRRVFC
SGEALPTALCREWEELTGAPLHNLYGPTEAAVDVSWYPACGPELAAVTGASVPIGWPVWN
TGLRILDASMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPYAPGERMYRTGDV
ARWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRAMSALPDVAQAVAHACVFNQAAATGGD
ARQLVGYLVSESGLPLDTEALKARLGEQLPPHMVPVVLIQLPALPLSANGKLDRKALPLP
TLSRVSRGRPPEPGVETTVASAFSQLLGCEVNDIDADFFALGGHSLLAMRLAATLGRELQ
RQVTPGQVMVASTVGKLSALLASDLSDEQAQRLGFDAILPLRESDGPTLFCFHPASGFAW
QFSVLARYLSPRWSIVGIQSPRPHGPMATAADLDAVCEHHLQTLLSQQPHGPYYLLGYSL
GGTLAQGIAARLRQRGEAVAFLGLLDTWPPETQNWAEKEANGLDPEVLAEIAREREAFLA
AQQGQASGELFSVIEGNYADAVRLLTTAHSAKFDGKATLFVAEKTRQPGMEPQRVWGPWV
GELEVFSQDCAHVDIISPQAFEAIGPVVREILGEG