Protein Info for BWI76_RS07550 in Klebsiella michiganensis M5al

Annotation: Zn-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 21 to 417 (397 residues), 431.5 bits, see alignment E=1.6e-133 PF04389: Peptidase_M28" amino acids 75 to 186 (112 residues), 30.4 bits, see alignment E=4.7e-11 PF01546: Peptidase_M20" amino acids 93 to 416 (324 residues), 103 bits, see alignment E=3.2e-33 PF07687: M20_dimer" amino acids 224 to 320 (97 residues), 28.2 bits, see alignment E=2.3e-10

Best Hits

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 88% identity to kpn:KPN_00569)

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.6, 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0I1 at UniProt or InterPro

Protein Sequence (422 amino acids)

>BWI76_RS07550 Zn-dependent hydrolase (Klebsiella michiganensis M5al)
MSSDVLFTPMTDTTDCRVNGERLWNSLMTLAEIGATPKGGCCRLTLTDLDRQGRDLVISW
AQAAGMRVTVDKIGNVFMRREGRNPALPPIVSGSHIDTQPTGGKFDGNYGVLAALEVVRT
LNDRDIETEAPIEVVFWTNEEGSRFVPVMMGSGVFAGVFPLETIYAAQDADGKTVGEELA
RIGYIGSQTPGDHPIGAYFEAHIEQGPILEDEEKMIGIVQGVLGIRWYDCVVTGQESHAG
PTPMRLRQDALQVATRIMQEVVAIAGRSDEGRGTVGMVQVYPNSRNVVPGEVTFSIDMRN
LSDALVDEMDRQLRAFIADVERESGLKIALKEVSHYPAAPFHPDCQAAIADAAQRLGYPA
REIVSGAGHDAVYMSYLAPTGMIFIPCKDGISHNEIEYASPEHVEAGANVLLQVMLQYAR
VV