Protein Info for BWI76_RS07340 in Klebsiella michiganensis M5al

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 TIGR01891: amidohydrolase" amino acids 16 to 379 (364 residues), 356.3 bits, see alignment E=1e-110 PF01546: Peptidase_M20" amino acids 75 to 388 (314 residues), 157.8 bits, see alignment E=3.7e-50 PF07687: M20_dimer" amino acids 188 to 285 (98 residues), 35.9 bits, see alignment E=6.5e-13

Best Hits

Swiss-Prot: 38% identical to YHAA_BACSU: Putative amidohydrolase YhaA (yhaA) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 92% identity to eae:EAE_13335)

MetaCyc: 38% identical to N-acetyl amino acid acetylase (Bacillus subtilis subtilis 168)
3.5.1.-

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0F0 at UniProt or InterPro

Protein Sequence (392 amino acids)

>BWI76_RS07340 amidohydrolase (Klebsiella michiganensis M5al)
MTDCVIPEIKATEDEMIAIRHYLHAHPELSLEEFNTSELVAGKLTEWGYTVTRGLAKTGV
VGTLSKGDSPRTIGLRADMDALPIFEATDLPWASTVAGKMHACGHDGHTTILLAAAKYIA
SPACQFNGTVHLIFQPAEEAIGGADLMIKDGLFERFPCERIFGLHNMPGLPVGKLGFYAG
NFMASADTVKITITGYGGHGAHPERTVDPIVTGAALVMNLQSIVARNVPPGATAVVSVGT
FQAGIASNVIPENVVMELSVRAMKPEIRDLLIKRIKELADFTAKSYGASSEVEVFDSYPV
LTNSDEETEFAKALALEVFGEEGVLESISPMNASEDFAFMLQKRPGCYFLLGNGEKGGKG
GCMVHNPGYDFNDDIISTGATLFVRLVETHCR