Protein Info for BWI76_RS07260 in Klebsiella michiganensis M5al

Annotation: branched chain amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 31 to 371 (341 residues), 204.9 bits, see alignment E=4.4e-64 PF01094: ANF_receptor" amino acids 51 to 369 (319 residues), 96 bits, see alignment E=3.5e-31 PF13433: Peripla_BP_5" amino acids 162 to 357 (196 residues), 28.6 bits, see alignment E=1.2e-10

Best Hits

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 94% identity to cko:CKO_02937)

Predicted SEED Role

"Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B098 at UniProt or InterPro

Protein Sequence (385 amino acids)

>BWI76_RS07260 branched chain amino acid ABC transporter substrate-binding protein (Klebsiella michiganensis M5al)
MKTVKISALSAAILLSGFASSAAWSAASETVIIGLAGPLTGPSARIGKDLENGAQLAIDD
INKQHPTIGGKAVTFKLQSEDDQSDPRTAVAVAQRLVDSGVAGVVGHWNTGTSIPAARVY
HDAGIAQVAPVATGHAYTKQGFDTSFRVMGHDDDGGQFAGQYAVQTLKAKRIAVIDDRTA
FGQGLADEFIKSLEVQGINIVDRQYVDDKTVDFSAVLTAIRSKNADLIFFGGVDSQAAPL
ARRIKQLGMNATLMGAGGFVSQTFLQLAQKEGDGVVALEPGLPVERMPGGKAFEQAYQSR
YHTHIELHAPFAYDATRVLVAAMEKADSVDPADYLPALRAINYAGVTGQIAFDKEGNLKS
PTFTVYKVVEGKWQPQTVLGGATTQ