Protein Info for BWI76_RS07250 in Klebsiella michiganensis M5al

Annotation: Zn-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01879: amidase, hydantoinase/carbamoylase family
  • amino acids 8 to 401 (394 residues), 397 bits, E=4.6e-123
• PF04389: Peptidase_M28
  • amino acids 65 to 142 (78 residues), 23.1 bits, E=5.4e-09
• PF01546: Peptidase_M20
  • amino acids 78 to 400 (323 residues), 94.2 bits, E=1.1e-30

Best Hits

Swiss-Prot: 45% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 79% identity to cko:CKO_02939)

MetaCyc: 45% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)
• thymine degradation (2/3 steps found)
• uracil degradation I (reductive) (2/3 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.6, 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

See A0A285B0D0 at UniProt or InterPro

Protein Sequence (413 amino acids)

>BWI76_RS07250 Zn-dependent hydrolase (Klebsiella michiganensis M5al)
MITVNGARLWSTLNEMALLGATPAGGVTRLALSEEDRQARDLLRQWAREAGFPCEVDSMG
NMFIRRAGKNSQLAPVLTGSHVDSQPLGGRYDGIYGVLAGLEALRTLNDRGIETERDIVL
VNWTNEEGARFAPAMLASGVWAGQFSEDFALARIDRDGISVSAALEAIGYRGECPAAAFP
LHACYEVHIEQGPILEEEGVDIGLVHAAMGQRWFNVTLEGFSAHAGTTPMGSRRDALTAF
AELALAVEHIGIAHNPDGRATIGMAQVIPGSRNVVPGRVACSVEFRHPQSPALEAMEREL
HRAAEALAARGVAANVERIFDYAPIAFDGECLARSQRAADELGYSARTMVSGAGHDTCYI
SKVAPASMIFIPCEKGISHNEAENILPEWAEKGANVLLNSLRLAADESACGAA