Protein Info for BWI76_RS06790 in Klebsiella michiganensis M5al

Annotation: mechanosensitive channel MscK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1113 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 493 to 514 (22 residues), see Phobius details amino acids 551 to 573 (23 residues), see Phobius details amino acids 580 to 600 (21 residues), see Phobius details amino acids 623 to 640 (18 residues), see Phobius details amino acids 652 to 673 (22 residues), see Phobius details amino acids 685 to 710 (26 residues), see Phobius details amino acids 722 to 744 (23 residues), see Phobius details amino acids 789 to 814 (26 residues), see Phobius details amino acids 834 to 854 (21 residues), see Phobius details amino acids 875 to 897 (23 residues), see Phobius details amino acids 908 to 935 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 44 to 283 (240 residues), 207.5 bits, see alignment E=6.3e-65 PF12794: MscS_TM" amino acids 503 to 820 (318 residues), 317.1 bits, see alignment E=3e-98 PF21088: MS_channel_1st" amino acids 881 to 922 (42 residues), 41.9 bits, see alignment (E = 1.9e-14) PF00924: MS_channel_2nd" amino acids 924 to 989 (66 residues), 86.2 bits, see alignment 3.3e-28 PF21082: MS_channel_3rd" amino acids 997 to 1080 (84 residues), 70.1 bits, see alignment 4.3e-23

Best Hits

Swiss-Prot: 85% identical to MSCK_ECOLI: Mechanosensitive channel MscK (mscK) from Escherichia coli (strain K12)

KEGG orthology group: K05802, potassium efflux system protein KefA (inferred from 85% identity to esc:Entcl_3325)

MetaCyc: 85% identical to potassium dependent, small conductance mechanosensitive channel (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-86

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AZQ6 at UniProt or InterPro

Protein Sequence (1113 amino acids)

>BWI76_RS06790 mechanosensitive channel MscK (Klebsiella michiganensis M5al)
MLHTIRWRNAISAVVLTLMFFIGCISVNPALAADLPERSEVQSQLATLNKQKDLTPQDKL
VQQDLTQTLETLDKIERIKSETTQLRQQVTQAPAKMNQAIDSLNALSDVPDDEATRKTLS
TLSLRQLESRVSQTLDDLQNAQNDLATYNSQLVSLQTQPERVQNAMYSASQQLQQIRNRL
NGTSTGEETLRPTQQNLLLAQQALLNAQIDQQRKSLEGNTVLQDTLQKQRDYVTAYSNRL
EHQLQLLQEAVNSKRLTLTEKTAQEAVSPDETARIQANPLVKQELDINHQLSERLISATE
NGNQLVQRNIKVKTWLDRALQSERDIKEQISVLKGSLLLSRILYQQQQTLPSAEELSDMT
NRIADLRLEQFEVNQQRDALFQSDAYVAKLEEGHSSEVNPEVHDALLEIVDMRRELLDQF
NKQLGNQLMMAINLQINQQQLMSVSTNLKAILTQQIFWVNSNRPMDWEWVKSFPEALKGQ
FKAMKITVNWEKAWPAVFIAFLAGLPLLLIAGLIRWRLNWLKAYQAKLASQVGQLRNDTQ
LHTPKAILIDLIRALPVVLVILAIGLILLTMQLNISDLLWAYSKKLALFWLVFGLCWKVL
EKDGVAVSHFNMPSQLTSHWRRQIVRVSLALLPLNFWSVMSELSPLHLMDDVLGQFVIFL
NLLLIAVLVLPMCRESWRDKESHSLRLITVTVLSIVPIALMVLTATGYFYTTLRLAGRWI
ETVYLVMLWNLLYQTVLRGLSVAARRIAWRRALARRQHLVKEGAEGAEPQEEPTIALEQV
NQQTLRITMLVMIALFAVLFWAIWSDLITVFAYLDSITLWHYNGTEAGVSAVKSVTMGSL
LFAIVASMVAWALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYAIIVIGAMTVFGSLG
VSWDKLQWLAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGTFSGTVSKIRI
RATTITDFDRKEVIIPNKAFVTERLINWSLSDTVTRVVIRLGVAYGSDLDKVKEVLLQAA
HEHPKVMQEPAPAVFFTTFGASTLDHELRLYVRELRDRSYAVDELNRAIDRLCRENNIDI
AFNQLEVHLRNEKGDEVTEVKRDKGGDASPTMG