Protein Info for BWI76_RS06605 in Klebsiella michiganensis M5al

Annotation: ammonium transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 32 to 56 (25 residues), see Phobius details amino acids 67 to 95 (29 residues), see Phobius details amino acids 117 to 141 (25 residues), see Phobius details amino acids 149 to 170 (22 residues), see Phobius details amino acids 183 to 204 (22 residues), see Phobius details amino acids 220 to 237 (18 residues), see Phobius details amino acids 248 to 268 (21 residues), see Phobius details amino acids 280 to 302 (23 residues), see Phobius details amino acids 307 to 324 (18 residues), see Phobius details amino acids 336 to 360 (25 residues), see Phobius details amino acids 376 to 396 (21 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 31 to 426 (396 residues), 454.7 bits, see alignment E=1.4e-140 PF00909: Ammonium_transp" amino acids 33 to 426 (394 residues), 427.9 bits, see alignment E=1.7e-132

Best Hits

Swiss-Prot: 89% identical to AMTB_ECOLI: Ammonia channel (amtB) from Escherichia coli (strain K12)

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 95% identity to kpu:KP1_1286)

MetaCyc: 89% identical to ammonium transporter (Escherichia coli K-12 substr. MG1655)
RXN-9615

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AZT3 at UniProt or InterPro

Protein Sequence (428 amino acids)

>BWI76_RS06605 ammonium transporter (Klebsiella michiganensis M5al)
MKIATMKTGLGALALLPGLAMAAPAVADKADNAFMMICTALVLFMSIPGIALFYGGLIRG
KNVLSMLTQVIVTFALVCVLWVVYGYTLAFGAGGSFFGNFDWVLLKNIELKALMGSFYQY
IHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWMTLSYVPIAHMVWGGGLLATHGA
LDFAGGTVVHINAAVAGLVGAYMMGKRVGFGKEAFKPHNLPMVFTGTAILYVGWFGFNAG
SASAANEIAGLAFVNTVVATAAAILAWTFGEWATRGKPSLLGACSGAIAGLVGITPACGY
IGVGGSLIVGIAAGLAGLWGVTVLKRWLRVDDPCDVFGVHGVCGIVGCILTGIFAATSLG
GVGYAEGVTMGHQVLVQLESIGITIVWSGVVAFIGYKVADMTVGLRVPEDQEREGLDVNS
HGENAYNA