Protein Info for BWI76_RS06570 in Klebsiella michiganensis M5al

Annotation: thiamin pyrimidine pyrophosphate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 PF00702: Hydrolase" amino acids 3 to 224 (222 residues), 29 bits, see alignment E=2e-10 PF05116: S6PP" amino acids 4 to 83 (80 residues), 25.5 bits, see alignment E=1.4e-09 amino acids 144 to 236 (93 residues), 48.3 bits, see alignment E=1.6e-16 TIGR00099: Cof-like hydrolase" amino acids 5 to 258 (254 residues), 202.2 bits, see alignment E=1.1e-63 TIGR01484: HAD hydrolase, family IIB" amino acids 5 to 229 (225 residues), 157.8 bits, see alignment E=4.3e-50 PF08282: Hydrolase_3" amino acids 6 to 258 (253 residues), 184 bits, see alignment E=6.9e-58

Best Hits

Swiss-Prot: 88% identical to COF_KLEP3: HMP-PP phosphatase (cof) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 88% identity to eae:EAE_12815)

MetaCyc: 79% identical to HMP-PP phosphatase (Escherichia coli K-12 substr. MG1655)
3.6.1.-; Pyridoxal phosphatase. [EC: 3.1.3.74]

Predicted SEED Role

"HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217)"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.74

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AZR0 at UniProt or InterPro

Protein Sequence (272 amino acids)

>BWI76_RS06570 thiamin pyrimidine pyrophosphate hydrolase (Klebsiella michiganensis M5al)
MARLAAFDMDGTLLMPDHRLGEKTLSTLKRLRARDITLTFATGRHALEMRHVIGEFSLDA
FLITGNGTRIHSLEGEELYRRDLAPDVAEEVLHSAWETSASMHFFNDGGWYTGQEIPEIL
KAHVFSGFRYQLYDPKRMPAHQVTKICFCGDHDDLRRLRIQLNEALGGRADLCFSAMDCL
EVLPGGCNKGAALAVLSQHLGLTLQDCMAFGDAMNDREMLGSVGRGVIMGNAMPQLKIEL
PHLPVIGDCRHQAVSHFLTHWLDNPDLPYSPE