Protein Info for BWI76_RS06365 in Klebsiella michiganensis M5al

Annotation: thiamine-monophosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01379: thiamine-phosphate kinase
  • amino acids 4 to 322 (319 residues), 381.3 bits, E=1.6e-118
• PF00586: AIRS
  • amino acids 28 to 136 (109 residues), 97.8 bits, E=5.2e-32
• PF02769: AIRS_C
  • amino acids 150 to 296 (147 residues), 39.5 bits, E=6.3e-14

Best Hits

Swiss-Prot: 85% identical to THIL_ECOLI: Thiamine-monophosphate kinase (thiL) from Escherichia coli (strain K12)

KEGG orthology group: K00946, thiamine-monophosphate kinase [EC: 2.7.4.16] (inferred from 90% identity to kva:Kvar_4012)

MetaCyc: 85% identical to thiamine monophosphate kinase (Escherichia coli K-12 substr. MG1655)
Thiamine-phosphate kinase. [EC: 2.7.4.16]

Predicted SEED Role

"Thiamine-monophosphate kinase (EC 2.7.4.16)" in subsystem Thiamin biosynthesis (EC 2.7.4.16)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiamine diphosphate salvage II (5/5 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiamine diphosphate salvage V (3/3 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage I (2/2 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.16

Sequence Analysis Tools

See A0A285AZL9 at UniProt or InterPro

Protein Sequence (325 amino acids)

>BWI76_RS06365 thiamine-monophosphate kinase (Klebsiella michiganensis M5al)
MACGEFSLIARYFDRVKSARLDVETGIGDDCALLNIPEKKTLAISTDTLVAGNHFLPDID
PADLAYKALAVNLSDLAAMGAEPAWLTLALTLPEADEAWLEAFSDSLFVQLNYYDMQLIG
GDTTRGPLSMTLGIHGFVPTGRGLKRSGAKPGDWIYVTGTPGDSAAGLAILQDRLHVQDN
ADADYLLARHLRPLPRVLQGQALRDLANSAIDLSDGLISDLGHILNASGCGARVDLDAMP
FSDAMLRQVETEQALRWALSGGEDYELCFTVPELNRGALDVALANLGARFTCIGQILPQS
EGLQFMREGKPVTFDFKGYDHFGAE