Protein Info for BWI76_RS06245 in Klebsiella michiganensis M5al

Annotation: proline-specific permease ProY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 transmembrane" amino acids 16 to 34 (19 residues), see Phobius details amino acids 40 to 62 (23 residues), see Phobius details amino acids 92 to 117 (26 residues), see Phobius details amino acids 124 to 142 (19 residues), see Phobius details amino acids 154 to 176 (23 residues), see Phobius details amino acids 197 to 220 (24 residues), see Phobius details amino acids 241 to 260 (20 residues), see Phobius details amino acids 277 to 298 (22 residues), see Phobius details amino acids 330 to 350 (21 residues), see Phobius details amino acids 356 to 377 (22 residues), see Phobius details amino acids 398 to 421 (24 residues), see Phobius details amino acids 427 to 445 (19 residues), see Phobius details PF03845: Spore_permease" amino acids 11 to 285 (275 residues), 29.8 bits, see alignment E=4.3e-11 PF00324: AA_permease" amino acids 15 to 451 (437 residues), 397.7 bits, see alignment E=1e-122 PF13520: AA_permease_2" amino acids 16 to 440 (425 residues), 142.7 bits, see alignment E=2.6e-45

Best Hits

Swiss-Prot: 94% identical to PROY_SALTY: Proline-specific permease ProY (proY) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K11736, proline-specific permease ProY (inferred from 94% identity to ecr:ECIAI1_0402)

MetaCyc: 49% identical to threonine/serine:H+ symporter ThrP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-71; TRANS-RXN-72

Predicted SEED Role

"Proline-specific permease proY" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AZH6 at UniProt or InterPro

Protein Sequence (456 amino acids)

>BWI76_RS06245 proline-specific permease ProY (Klebsiella michiganensis M5al)
MESSNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGVAAYIIMR
ALGEMSVHNPAASSFSRYAQDYLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPA
VPHWVWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMILAGFGIIIWGIGNGGQ
PTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTNGSPFVLTFQHLGITFAASILNFVVLTASLSAINS
DVFGVGRMLHGMAEQGSAPKMFAKTSRRGVPWVTVMVMTIALLFAVYLNYIMPENVFLVI
ASLATFATVWVWIMILLSQIAFRRRLPPEEAKALKFKVPGGVTTTVIGLLFLVFIIGLIG
YHPDTRISLYVGFAWIILLLIGWQFKTRRERKLAKA