Protein Info for BWI76_RS06170 in Klebsiella michiganensis M5al
Annotation: pyrroline-5-carboxylate reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to P5CR_ECOLI: Pyrroline-5-carboxylate reductase (proC) from Escherichia coli (strain K12)
KEGG orthology group: None (inferred from 96% identity to eae:EAE_12380)MetaCyc: 91% identical to pyrroline-5-carboxylate reductase (Escherichia coli K-12 substr. MG1655)
Pyrroline-5-carboxylate reductase. [EC: 1.5.1.2]
Predicted SEED Role
"Pyrroline-5-carboxylate reductase (EC 1.5.1.2)" in subsystem Proline Synthesis (EC 1.5.1.2)
MetaCyc Pathways
- L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
- L-proline biosynthesis III (from L-ornithine) (2/3 steps found)
- L-arginine degradation XIII (reductive Stickland reaction) (3/5 steps found)
- L-arginine degradation VI (arginase 2 pathway) (2/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.5.1.2
Use Curated BLAST to search for 1.5.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AZI4 at UniProt or InterPro
Protein Sequence (269 amino acids)
>BWI76_RS06170 pyrroline-5-carboxylate reductase (Klebsiella michiganensis M5al) MEKKIGFIGCGNMGKAILGGLIASGQVQPGQIWVYTPSPDKVAALHDQYGINAAQSAQEV AQVADIVFGAVKPGVMVKVLSDIASSLNKDSLVVSIAAGVTLDQLARALGHDRKIVRAMP NTPSLVNAGMTSVTPNALVSAEETAEILTIFRCFGQAEIIAEPMIHPVVGVSGSAPAYVF MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLESGEHPGALKDMVCSPGGTTIEAV RVLEEKGFRSAVIEAMVKCMEKSEKLSRS