Protein Info for BWI76_RS05755 in Klebsiella michiganensis M5al

Annotation: beta-ketoacyl-[acyl-carrier-protein] synthase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 56% identical to FABF_RHIME: 3-oxoacyl-[acyl-carrier-protein] synthase 2 (fabF) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K09458, 3-oxoacyl-[acyl-carrier-protein] synthase II [EC: 2.3.1.179] (inferred from 92% identity to kpu:KP1_2826)

MetaCyc: 46% identical to (5Z)-3-oxo-dodec-5-enoyl-[acp] synthase (Enterococcus faecalis)
Beta-ketoacyl-acyl-carrier-protein synthase II. [EC: 2.3.1.179, 2.3.1.41]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179)" (EC 2.3.1.179)

MetaCyc Pathways

superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
superpathway of unsaturated fatty acids biosynthesis (E. coli) (20/20 steps found)
superpathway of fatty acid biosynthesis I (E. coli) (16/16 steps found)
biotin biosynthesis I (15/15 steps found)
8-amino-7-oxononanoate biosynthesis I (11/11 steps found)
oleate biosynthesis IV (anaerobic) (13/14 steps found)
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
(5Z)-dodecenoate biosynthesis I (6/6 steps found)
cis-vaccenate biosynthesis (5/5 steps found)
fatty acid elongation -- saturated (5/5 steps found)
superpathway of fatty acid biosynthesis initiation (5/5 steps found)
gondoate biosynthesis (anaerobic) (4/4 steps found)
(5Z)-dodecenoate biosynthesis II (5/6 steps found)
stearate biosynthesis II (bacteria and plants) (5/6 steps found)
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
palmitate biosynthesis III (21/29 steps found)
fatty acid biosynthesis initiation (mitochondria) (3/4 steps found)
stearate biosynthesis IV (4/6 steps found)
anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
petroselinate biosynthesis (2/6 steps found)
streptorubin B biosynthesis (20/34 steps found)
mycolate biosynthesis (25/205 steps found)
superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.179, 2.3.1.41

Use Curated BLAST to search for 2.3.1.179 or 2.3.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AZ85 at UniProt or InterPro

Protein Sequence (423 amino acids)

>BWI76_RS05755 beta-ketoacyl-[acyl-carrier-protein] synthase II (Klebsiella michiganensis M5al)
MGVSVKRIVITGMGIVSPLGCGVQHVWQSLLAGKSGITKLSEQLVADIPCKVAGLVPSIE
HDALHGFDPLAIIPAKERKKMDRFIEFALVAAHEALTQARWSPESATEQERTATVIATGI
GGFGEIANAVHTTDERGPRRLSPFTIPSFLANLAAGHVSIAHGFRGPIGAPVTACAAGAQ
AIGDAARMIHSGEADIALCGGAEAAIHRVSLAGFAAAKALSSSSNDHPEAASRPFDRDRD
GFVMGEGAGLIVIESLEHALARGATPLAELVGYGTSADAYHLTAGPEDGDGARRAMEMAI
RQAGISAEEIDHINAHATSTQVGDKGELAAIKKLFGAHPVAITSTKSATGHLLGAAGGIE
AIFTIQALRDQMVPPTLNLHHPDENAEGLNLVALVARPQKMRYALSNGFGFGGVNASLLL
KRW