Protein Info for BWI76_RS05340 in Klebsiella michiganensis M5al

Annotation: proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 567 (567 residues), 922.7 bits, E=5.3e-282
• PF00587: tRNA-synt_2b
  • amino acids 97 to 459 (363 residues), 128.6 bits, E=4.5e-41
• PF04073: tRNA_edit
  • amino acids 257 to 376 (120 residues), 95.8 bits, E=3.1e-31
• PF03129: HGTP_anticodon
  • amino acids 475 to 567 (93 residues), 72.4 bits, E=4.1e-24

Best Hits

Swiss-Prot: 97% identical to SYP_KLEP7: Proline--tRNA ligase (proS) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 97% identity to kpn:KPN_00208)

MetaCyc: 94% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• anguibactin biosynthesis (2/8 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• pyochelin biosynthesis (2/11 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See A0A285AYW9 at UniProt or InterPro

Protein Sequence (572 amino acids)

>BWI76_RS05340 proline--tRNA ligase (Klebsiella michiganensis M5al)
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
MNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGERPFVLGPTHEEVITDLIRN
ELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVIFSDSSDYAANIEFAEAVA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEDSKSPLIALLVRG
DHELNEVKAEKLPQVASPLTFATEEEIRALVQAGPGSLGPVNMPIPVVIDRTVAAMSDFA
AGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGKGTLLIKRGIEVGHIFQLGTK
YSEAMKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDDRGILWPEAIAPFQVAILP
MNMHKSYRVQELAEKLYAELTALGIDVLMDDRKERPGVMFADMELIGIPHTIVLGDRNLD
NDDIEYKYRRSGEKQLIKTGDIVNYLVNAIKG