Protein Info for BWI76_RS05330 in Klebsiella michiganensis M5al
Annotation: aminoacyl-tRNA hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to ARFB_ECOLI: Peptidyl-tRNA hydrolase ArfB (arfB) from Escherichia coli (strain K12)
KEGG orthology group: K15034, ribosome-associated protein (inferred from 93% identity to kpe:KPK_4529)MetaCyc: 79% identical to peptidyl-tRNA hydrolase, ribosome rescue factor (Escherichia coli K-12 substr. MG1655)
Aminoacyl-tRNA hydrolase. [EC: 3.1.1.29]
Predicted SEED Role
"Hypothetical protein YaeJ with similarity to translation release factor"
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.29
Use Curated BLAST to search for 3.1.1.29
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AZ46 at UniProt or InterPro
Protein Sequence (137 amino acids)
>BWI76_RS05330 aminoacyl-tRNA hydrolase (Klebsiella michiganensis M5al) MITISRSVMIADDEVSLSGIRAQGAGGQHVNKASTAIHLRFDIKASSLPEFYKERLLTSS HHLISADGVVIIKAQEYRSQEMNREAAIARLVALIQELTAVQKSRRETRPTRASKERRLA SKAQKSSVKALRGKVRH