Protein Info for BWI76_RS05170 in Klebsiella michiganensis M5al

Annotation: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 2 to 226 (225 residues), 180.1 bits, E=2.3e-57
• TIGR01704: MTA/SAH nucleosidase
  • amino acids 2 to 229 (228 residues), 422.6 bits, E=1.8e-131

Best Hits

Swiss-Prot: 92% identical to MTNN_KLEP7: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 90% identity to eco:b0159)

MetaCyc: 90% identical to adenosylhomocysteine nucleosidase (Escherichia coli ATCC 25922)
Methylthioadenosine nucleosidase. [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) / S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" in subsystem Adenosyl nucleosidases or Methionine Salvage or Polyamine Metabolism or Methionine Biosynthesis or Methionine Degradation (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine salvage cycle II (plants) (9/11 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (5/6 steps found)
• S-methyl-5'-thioadenosine degradation I (2/2 steps found)
• autoinducer AI-2 biosynthesis I (4/5 steps found)
• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• 5'-deoxyadenosine degradation II (3/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (5/7 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Sequence Analysis Tools

See A0A285AYR9 at UniProt or InterPro

Protein Sequence (232 amino acids)

>BWI76_RS05170 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Klebsiella michiganensis M5al)
MKIGIIGAMEEEVTLLRDKIDNRQTLTIGGSEIYTGQLQGIDVALLKSGIGKVAAAMGAT
LLMEHCKPDVIINTGSAGGLASTLKVGDIVVSDETRYHDADVTAFGYEYGQLPGCPAGFK
ADDKLIAAAETCIKALNLNAVRGLIVSGDAFINGSIGLAKIRHNFPQAVAVEMEATAIAH
VCHNYGVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSTLMVENLVQHLARG