Protein Info for BWI76_RS05100 in Klebsiella michiganensis M5al

Annotation: pullulanase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1090 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF03714: PUD" amino acids 76 to 168 (93 residues), 36.3 bits, see alignment E=2.7e-12 TIGR02103: alpha-1,6-glucosidases, pullulanase-type" amino acids 181 to 1077 (897 residues), 1409.2 bits, see alignment E=0 PF17967: Pullulanase_N2" amino acids 184 to 293 (110 residues), 124.9 bits, see alignment E=8.5e-40 PF02922: CBM_48" amino acids 304 to 388 (85 residues), 63.9 bits, see alignment 4.2e-21 PF18494: Pullulanase_Ins" amino acids 489 to 562 (74 residues), 101.9 bits, see alignment 6.3e-33 PF11852: Pullul_strch_C" amino acids 913 to 1073 (161 residues), 150.3 bits, see alignment E=1.1e-47

Best Hits

Swiss-Prot: 100% identical to PULA_KLEPN: Pullulanase (pulA) from Klebsiella pneumoniae

KEGG orthology group: None (inferred from 90% identity to kva:Kvar_4220)

Predicted SEED Role

"alpha-dextrin endo-1,6-alpha-glucosidase precursor (pullulanase precursor)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYV8 at UniProt or InterPro

Protein Sequence (1090 amino acids)

>BWI76_RS05100 pullulanase (Klebsiella michiganensis M5al)
MLRYTRNALVLGSLVLLSGCDNGSSSSSSGNPDTPDNQDVVVRLPDVAVPGEAVTAVENQ
AVIHLVDIAGITSSSAADYSSKNLYLWNNETCDALSAPVADWNDVSTTPSGSDKYGPYWV
IPLNKESGCINVIVRDGTDKLIDSDLRVAFGDFTDRTVSVIAGNSAVYDSRADAFRAAFG
VALAEAHWVDKNTLLWPGGQDKPIVRLYYSHSSKVAADGEGKFTDRYLKLTPTTVSQQVS
MRFPHLSSYAAFKLPDNANVDELLQGETVAIAAAEDGILISATQVQTAGVLDDAYAEAAE
ALSYGAQLADGGVTFRVWAPTAQQVDVVVYSADKKVIGSHPMTRDSASGAWSWQGGSDLK
GAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNSEYSQVVDLNDSALKPDGWDNLTMPH
AQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAGDSNMVQHLKTLSASGVTH
VELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAGYCDSGSTVEEVLNQLKQ
SDSQDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTTRIKEFRTMIQAIKQ
DLGMNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDSAPEHRM
FAKLIADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPDIYFFGEGWDSNQS
DRFEIASQINLKGTGIGTFSDRLRDSVRGGGPFDSGDALRQNQGIGSGAGVLPNELASLS
DDQVRHLADLTRLGMAGNLADFVMIDKDGAAKKGSEIDYNGAPGGYAADPTEVVNYVSKH
DNQTLWDMISYKASQEADLATRVRMQAVSLATVMLGQGIAFDQQGSELLRSKSFTRDSYD
SGDWFNRVDYSLQDNNYNVGMPRISDDGSNYEVITRVKEMVATPGEAELKQMTAFYQELT
ELRKSSPLFTLGDGSAVMKRVDFRNTGSDQQAGLLVMTVDDGMKAGASLDSRLDGLVVAI
NAAPESRTLNEFAGETLQLSAIQQTAGENSLANGVQIAADGTVTLPAWSVAVLELPQGEA
QGAGLPVSSK