Protein Info for BWI76_RS05060 in Klebsiella michiganensis M5al

Annotation: type II secretion system protein GspJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 198 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF07963: N_methyl" amino acids 3 to 27 (25 residues), 30.1 bits, see alignment (E = 2.6e-11) TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 6 to 28 (23 residues), 25.2 bits, see alignment (E = 1.1e-09) TIGR01711: type II secretion system protein J" amino acids 7 to 192 (186 residues), 194.7 bits, see alignment E=1.4e-61 PF11612: T2SSJ" amino acids 58 to 190 (133 residues), 143.5 bits, see alignment E=5.7e-46

Best Hits

Swiss-Prot: 100% identical to GSPJ_KLEPN: Type II secretion system protein J (pulJ) from Klebsiella pneumoniae

KEGG orthology group: K02459, general secretion pathway protein J (inferred from 71% identity to kpe:KPK_4582)

Predicted SEED Role

"General secretion pathway protein J"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYW8 at UniProt or InterPro

Protein Sequence (198 amino acids)

>BWI76_RS05060 type II secretion system protein GspJ (Klebsiella michiganensis M5al)
MIRRSSGFTLVEMLLALAILAALSVAAVTVLQNVMRADTLTRDKSGRMQALQQTFSQMAA
DFSQIIPRRSRDSASLFFAGRFQLGSDDGAIAFSRNGWPNPLGLLPRSEIQNVSYRLRGS
QLERLNYDQQDPLPGSQPTVTIVLRDVRAFGLRFYASGRWQDEWQQAQTLPQGLEVTLTL
EPYGEIRRLFLLTPGDSR