Protein Info for BWI76_RS04945 in Klebsiella michiganensis M5al

Annotation: multicopper oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF07732: Cu-oxidase_3" amino acids 56 to 166 (111 residues), 99.9 bits, see alignment E=1.6e-32 PF00394: Cu-oxidase" amino acids 227 to 289 (63 residues), 23.6 bits, see alignment E=7.5e-09 PF07731: Cu-oxidase_2" amino acids 404 to 527 (124 residues), 85.3 bits, see alignment E=5.3e-28

Best Hits

Swiss-Prot: 76% identical to CUEO_SALTY: Blue copper oxidase CueO (cueO) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 87% identity to eae:EAE_11445)

MetaCyc: 77% identical to multicopper oxidase CueO (Escherichia coli K-12 substr. MG1655)
RXN0-2945 [EC: 1.16.3.4]; Ferroxidase. [EC: 1.16.3.4, 1.16.3.1]; Laccase. [EC: 1.16.3.4, 1.16.3.1, 1.10.3.2]; RXN0-2943 [EC: 1.16.3.4, 1.16.3.1, 1.10.3.2]

Predicted SEED Role

"Blue copper oxidase CueO precursor" in subsystem Copper homeostasis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.16.3.1

Use Curated BLAST to search for 1.10.3.2 or 1.16.3.1 or 1.16.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYN1 at UniProt or InterPro

Protein Sequence (527 amino acids)

>BWI76_RS04945 multicopper oxidase (Klebsiella michiganensis M5al)
MQRREFLKLTAAVSVASALPLWSRSVFAASRPSLPVPELLTADARNRISLIIQAGKTQFG
SHLATTWGYNGNLLGPALQLKQGEQATIDIHNRLAEETTVHWHGLEVPGAVDGGPHGVIA
AGGSRTVTFTPTQRAATCWFHPHQHGKTGRQVAMGLAGLVLISDAESEKLLLPKQWGIDD
VPVIVQDKKFTAAGEIDYQLDIMSAAVGWFGDTLLTNGALYPEHAAPRGWLRLRLLNGCN
ARSLNFATSDKRPMYVVASDGGLLAEPVKVNELPVLMGERFEVLVDTTDGKPFDLITLPV
NQMGMAIAPFDRPQPVLRVQPLTIQASGKLPDVLAAMPALPSLQGFTQRTLQLSMDPMLD
MMGMQALMQKYGDQAMSGMDHGMMMNHGDMGNMNHGNMNHGGMAQMKHGHQGFDFHNANR
INGKAFDMNVPLFAAAKGQYERWVISGKGDMMLHPFHIHGTQFRILSENGKAPAPHRSGW
KDTVRVEGDVSEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV