Protein Info for BWI76_RS04735 in Klebsiella michiganensis M5al

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 transmembrane" amino acids 305 to 327 (23 residues), see Phobius details TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 22 to 491 (470 residues), 548.7 bits, see alignment E=6.3e-169 PF01225: Mur_ligase" amino acids 25 to 102 (78 residues), 41 bits, see alignment E=3e-14 PF08245: Mur_ligase_M" amino acids 114 to 318 (205 residues), 182.8 bits, see alignment E=1.1e-57 PF02875: Mur_ligase_C" amino acids 339 to 425 (87 residues), 102.4 bits, see alignment E=2.1e-33

Best Hits

Swiss-Prot: 92% identical to MURE_SHIFL: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Shigella flexneri

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 96% identity to kpn:KPN_00089)

MetaCyc: 92% identical to UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. [EC: 6.3.2.13]

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYH8 at UniProt or InterPro

Protein Sequence (495 amino acids)

>BWI76_RS04735 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Klebsiella michiganensis M5al)
MADRNLRDLLAPWVPNAPERILREMTLDSRVAASGDLFVAVLGHQADGRRYIPQAIAQGV
AAIIAEAKDEAHDGEIREMHGVPVIYLSQLNERLSALAGRFYHQPSEQLRLVGVTGTNGK
TTTTQLLAQWAQLLGETSAVMGTVGNGLLEKVIPTENTTGSAVDVQHVLSGLAGQGATFA
AMEVSSHGLVQHRVAALQFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSTHHCGQAIVN
ADDEVGRRWLAKLPDAVAVSMEDHINPNCRGRWLKATAVNYHDSGATIQFASSWGNGEIE
SRLMGAFNVSNLLLALATLLALGYPLAELLKTAARLQPVCGRMEVFSAPGKPTVVVDYAH
TPDALEKALQAARLHCNGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTEE
PRAIINDILAGMLDAGHAKAVEGRAEAVTNAVMQAKENDVVLVAGKGHEDYQIVGNRRLD
YSDRVTVARLLGAVA