Protein Info for BWI76_RS04660 in Klebsiella michiganensis M5al

Annotation: 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 PF00724: Oxidored_FMN" amino acids 7 to 336 (330 residues), 246.4 bits, see alignment E=2.8e-76 PF01134: GIDA" amino acids 385 to 418 (34 residues), 28.5 bits, see alignment (E = 4.8e-10) PF07992: Pyr_redox_2" amino acids 385 to 621 (237 residues), 86.6 bits, see alignment E=1.2e-27 PF00890: FAD_binding_2" amino acids 386 to 424 (39 residues), 21.7 bits, see alignment 6.1e-08 PF12831: FAD_oxidored" amino acids 386 to 424 (39 residues), 32 bits, see alignment 4.7e-11 PF00070: Pyr_redox" amino acids 519 to 586 (68 residues), 35.4 bits, see alignment E=6.9e-12

Best Hits

KEGG orthology group: None (inferred from 88% identity to eae:EAE_11165)

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYM7 at UniProt or InterPro

Protein Sequence (677 amino acids)

>BWI76_RS04660 2,4-dienoyl-CoA reductase (Klebsiella michiganensis M5al)
MSHYPHLFSPLTINGMTIKNRIIMPPMGTNLASLNGEVTQEQIDYYELRARGGTGLITIE
NICIDFPFASNGTTQLRIDNDQYIPRLFKLTETLHKHGACVGIQLNHAGASAYAYRLNGE
MPLSSSSTPSKKNGNIPRPMTHDEIYHAVDKFGDAAERVRRAGFDCVEIHAGHSYLISQF
LSPLFNKRTDEFGGSVENRARILTLIVDKVRACVGPRFPVSLRISADDFLEGGNTLEDSL
RILELCEEKVDIINVSAAQNDNLNFQIDQMSLEDGWKRYLSRAVRDKFHKPTVIAGNIRT
PKVAEDIIASGDADLIAIGRGLIAEPEWVQKVQSGNERLMRKCISCNIGCADHRIARSRP
LRCSINPDIIHGDAYKQQRVIRDTNVVVIGAGTAGMEAACTAAEVGCHTWLLEEKHSIGG
LASEISLLPEKKRIADFPQFMKNRIASLDNLMLQVGKRATVASVSALRPNLIVNATGSVP
LLPPIEGLRENIDVEGGNVFSITNMIHHLDQFADATGKRIAVVGAGAVGLDVIEYFSARG
ASAVLIEMQDAPGRDLDIITKNAMLTMLDEHHVEQHMRTQLVAVTPTHFTVKNADGAFDI
PFDYGFVCLGMRANTAGLNEIEQWARANNVKIMNIGDSLMARRIIDGVREGRNVLDTLED
MGALGNRESTKIPFLTY