Protein Info for BWI76_RS04570 in Klebsiella michiganensis M5al

Annotation: RNA polymerase-associated protein RapA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 968 PF18339: Tudor_1_RapA" amino acids 3 to 53 (51 residues), 83.5 bits, see alignment 2.8e-27 PF18337: Tudor_RapA" amino acids 55 to 118 (64 residues), 89.5 bits, see alignment 4.6e-29 PF04851: ResIII" amino acids 168 to 317 (150 residues), 43.6 bits, see alignment E=1.1e-14 PF00270: DEAD" amino acids 172 to 317 (146 residues), 28.4 bits, see alignment E=4.6e-10 PF00176: SNF2-rel_dom" amino acids 173 to 442 (270 residues), 70.1 bits, see alignment E=6.4e-23 PF00271: Helicase_C" amino acids 491 to 601 (111 residues), 67.3 bits, see alignment E=4.8e-22 PF12137: RapA_C" amino acids 605 to 965 (361 residues), 515.9 bits, see alignment E=2.1e-158

Best Hits

Swiss-Prot: 92% identical to RAPA_CITK8: RNA polymerase-associated protein RapA (rapA) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K03580, ATP-dependent helicase HepA [EC: 3.6.4.-] (inferred from 92% identity to cko:CKO_03323)

Predicted SEED Role

"RNA polymerase associated protein RapA (EC 3.6.1.-)" (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.-

Use Curated BLAST to search for 3.6.1.- or 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYL7 at UniProt or InterPro

Protein Sequence (968 amino acids)

>BWI76_RS04570 RNA polymerase-associated protein RapA (Klebsiella michiganensis M5al)
MPFTLGQRWISDTESELGLGTVVALDARMVTLLFPAIGENRLYARNDSPITRVMFNPGDT
ITSHEGWQLQVDEVNEENGLLAYTGTRLDTQEANVTLREVLLDSKLVFSKPQDRLFAGQI
DRMDRFALRYRARKFQSEQYRMPWSGLRGQRTSLIPHQLNIAHDVGRRHAPRVLLADEVG
LGKTIEAGMILHQQLLSGAAERVLIVVPETLQHQWLVEMLRRFNLRFSLFDDERYAEAQH
DAINPFETEQLVICSLDFVRRSKQRLEHLCDAEWDLMVVDEAHHLVWSEDAPSREYQAIE
QLAERVPGILLLTATPEQLGMESHFARLRLLDPNRFHDFAQFVEEQQNYRPVADAVALLL
AGNKLNDAELNALSDLIGEQDIEPLLQAANSDRDDAQAARQELVSMLMDRHGTSRVLFRN
TRNGVKGFPKRELHTIRLPLPTQYQTAIKVSGIMGARKTAEERARDMLYPEQIYQEFEGD
TGTWWNFDPRVEWLMGHLTSHRSQKVLVICAKAATALQLEQVLREREGIRAAVFHEGMSI
IERDRAAAWFAEEDTGAQVLLCSEIGSEGRNFQFASNLVMFDLPFNPDLLEQRIGRLDRI
GQAHDIQIHVPYLERTAQSVLVRWYHEGLDAFEHTCPTGRTVYDSVHNELINYLAAPENT
DGFDELIKACRQQHDALKAQLEQGRDRLLEIHSNGGEKAQQLAESIEEQDDDTSLIAFSM
NLFDIVGINQDDRGENLIVLTPSDHMLVPDFPGLPEDGCTITFERDVALSREDAQFITWE
HPLIINGLDLILSGDTGSSTISLLKNKALPVGTLLLELIYVVEAQAPKHLQLNRFLPPTP
VRMLLDKNGNNLAGQVEFESFNRQLSAVNRHTGSKLVNAVQQDVHAILQQGEGQVAKAAQ
ALIDAARKEADDKLTAELSRLEALRAVNPNIRDDELAAIESNRQQVMDALAQAGWRLDAL
RLIVVTHQ