Protein Info for BWI76_RS04535 in Klebsiella michiganensis M5al

Annotation: LPS assembly protein LptD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 780 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF03968: LptD_N" amino acids 53 to 194 (142 residues), 85.9 bits, see alignment E=2.4e-28 PF04453: LptD" amino acids 309 to 693 (385 residues), 389.8 bits, see alignment E=1.7e-120

Best Hits

Swiss-Prot: 82% identical to LPTD_ECOK1: LPS-assembly protein LptD (lptD) from Escherichia coli O1:K1 / APEC

KEGG orthology group: None (inferred from 92% identity to eae:EAE_11040)

MetaCyc: 82% identical to lipopolysaccharide assembly protein LptD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-237 [EC: 7.5.2.5]

Predicted SEED Role

"Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYF6 at UniProt or InterPro

Protein Sequence (780 amino acids)

>BWI76_RS04535 LPS assembly protein LptD (Klebsiella michiganensis M5al)
MKKRIPSLLATMIASALYSQQGLAADLATQCMLGVPSYNRPLVEGKPNDLPVTIDADHAK
GNYPDNAVFTGNVDINQGNSRLQADEVQLHQQQADGQPQPVRTVDALGNVHYDDNQVILK
GPKAWSNLNTKDTNVWQGDYQMVGRQGRGTADLMKQRGENRYTILENGSFTSCLPGSDTW
SVVGSEVIHDREEQVAEIWNARFKLGSVPIFYSPYLQLPVGDKRRSGFLIPNAKYSTKNG
AEFYLPYYWNIAPNFDATLTPHYMSKRGGVMWENEFRYLTKAGTGLIEFDYLPSDKIYQD
DHSGDSNSRRWLFYWNHSGVVDQVWRFNADYTKVSDPDYFNDFSSKYGSSTDGYATQKLS
AGYANQNFDATVSTKQFQVFNRDSSNAYSAEPQLDVNYYQNDVGPFDTHLYGQAVHFVNS
NSRMPEATRVHFEPTINLPLSNGWGSINTEAKLLATHYQQSNLDDYNAANGTDYKDSVNR
VMPQFKVDGKMVFERDMQENYTQTLEPRMQYLYIPYRDQSKIGTYDSTLLQSDYSGLFRD
RSYSGLDRIASANQVSTGITSRVYDAAAVERFNISVGQIYYFTEARTGDDNINWENNDTR
GSLVWAGDTYWRITDDWGLRGGIQYDTRLDNVATGSGTVEYRRDENRLVQLNYRYASPEY
IQATLPSYSTAKQYKDGISQVGMTASWPIVDRWAVVGAYYYDTNTRKSANQMLGVQYSSC
CYAIRVGYERKINGWDNSSSESKYDNTFGFNIELRGLSSNYGLGTQQMLRSNILPYQSSL