Protein Info for BWI76_RS04525 in Klebsiella michiganensis M5al
Annotation: 4-hydroxythreonine-4-phosphate dehydrogenase PdxA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to PDXA_CITK8: 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
KEGG orthology group: K00097, 4-hydroxythreonine-4-phosphate dehydrogenase [EC: 1.1.1.262] (inferred from 91% identity to kva:Kvar_4334)MetaCyc: 85% identical to 4-hydroxythreonine-4-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
4-hydroxythreonine-4-phosphate dehydrogenase. [EC: 1.1.1.262]
Predicted SEED Role
"4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.262)
MetaCyc Pathways
- superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
- pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.262
Use Curated BLAST to search for 1.1.1.262
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AYK8 at UniProt or InterPro
Protein Sequence (329 amino acids)
>BWI76_RS04525 4-hydroxythreonine-4-phosphate dehydrogenase PdxA (Klebsiella michiganensis M5al) MKCMNRVVITPGEPAGIGPDLVVQLAQRDWPVELVICADGALLSDRAKLLGLPLSLLPYD PTAPATPQRAGTLTLLPVALNAPVEPGVLNVSNGRYVVDTLARACDGCLNGEFAALITGP VHKGIINDAGIAFTGHTEFFEERSQASKVVMMLATEALRVALVTTHLPLKAISDAITPEL LREIITILDHDLRTKFGISNPHVLVCGLNPHAGEGGHMGTEEIDTIIPVLEEMRAKGMNL SGPLPADTLFQPKYLDHADAVLAMYHDQGLPVLKYQGFGRGVNITLGLPFIRTSVDHGTA LELAGQGKAEVGSFITALNLAIKMIVNTQ