Protein Info for BWI76_RS04450 in Klebsiella michiganensis M5al

Annotation: citrate lyase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 TIGR01584: citrate lyase, alpha subunit" amino acids 36 to 505 (470 residues), 810.2 bits, see alignment E=3.1e-248 PF04223: CitF" amino acids 40 to 504 (465 residues), 844.8 bits, see alignment E=1.1e-258

Best Hits

Swiss-Prot: 93% identical to CILA_KLEPN: Citrate lyase alpha chain (citF) from Klebsiella pneumoniae

KEGG orthology group: K01643, citrate lyase subunit alpha / citrate CoA-transferase [EC: 2.8.3.10 4.1.3.6] (inferred from 93% identity to efe:EFER_0035)

MetaCyc: 93% identical to citrate lyase alpha subunit (Klebsiella pneumoniae)
Citrate (pro-3S)-lyase. [EC: 4.1.3.6]; Citrate CoA-transferase. [EC: 4.1.3.6, 2.8.3.10]

Predicted SEED Role

"Citrate lyase alpha chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 2.8.3.10 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYD0 at UniProt or InterPro

Protein Sequence (508 amino acids)

>BWI76_RS04450 citrate lyase subunit alpha (Klebsiella michiganensis M5al)
MKETVAMLNQQYVMPEGLAPYAGVTAKSPWLASESEKRQRKICASLEEAIRRSGLQNGMT
ISFHHAFRGGDKVVNMVVAKLAEMGFRDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGL
RGKLGEEISAGLMENPVQIHSHGGRAYLVQSGELNIDVAFLGVPCCDEYGNANGFSGKSR
CGSLGYAKVDADAAKCVVLLTEEWVDYPNYPASIAQDQVDLIVQVDEVGDPAKITAGAIR
LTSNPRELLIARQAAKVIEHSGYFKDGFSLQTGTGGASLAVTRFLEDKMRRHNITASFGL
GGITGTMVDLHEKGLIKALLDTQSFDGDAARSLANNPNHIEISANQYANPGSKGIACERL
NVVMLSALEIDTGFNVNVMTGSNGVLRGASGGHCDTAAGADLTIITAPLVRGRIPCVVEK
VLTRVTPGASVDVLVTDHGIAVNPARQDLIDRLRQADIPLMTIEALQQRAELLTGKPEPI
AFTDRVVAVVRYRDGSVIDVIHQVKSAG