Protein Info for BWI76_RS04445 in Klebsiella michiganensis M5al

Annotation: citrate (pro-3S)-lyase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01588: citrate (pro-3S)-lyase, beta subunit" amino acids 2 to 287 (286 residues), 505.3 bits, see alignment E=2e-156 PF03328: HpcH_HpaI" amino acids 5 to 223 (219 residues), 269.4 bits, see alignment E=1.9e-84

Best Hits

Swiss-Prot: 96% identical to CITE_KLEPN: Citrate lyase subunit beta (citE) from Klebsiella pneumoniae

KEGG orthology group: None (inferred from 96% identity to eae:EAE_10955)

MetaCyc: 96% identical to citrate lyase beta subunit (Klebsiella pneumoniae)
Citryl-CoA lyase. [EC: 4.1.3.34, 4.1.3.6]

Predicted SEED Role

"Citrate lyase beta chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYL4 at UniProt or InterPro

Protein Sequence (289 amino acids)

>BWI76_RS04445 citrate (pro-3S)-lyase subunit beta (Klebsiella michiganensis M5al)
MKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDTARLLVHHALQHPLYRD
VETVVRINPLNTPFGLADLEAVVRAGVDMVRLPKTDSKEDIHELEAHVERIERECGREVG
STKLMAAIESALGVVNAVEIARSSPRLSAIALAAFDYVMDMGTCRGDGTELFYARCAVLH
AARVAGIAAYDVVWSDINNEEGFLAEANLAKNLGFNGKSLVNPRQIELLHQVYAPTLKDV
EHARDVIAAAEEAEERGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD