Protein Info for BWI76_RS04430 in Klebsiella michiganensis M5al

Annotation: citrate carrier protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 47 to 68 (22 residues), see Phobius details amino acids 78 to 97 (20 residues), see Phobius details amino acids 115 to 133 (19 residues), see Phobius details amino acids 144 to 170 (27 residues), see Phobius details amino acids 176 to 193 (18 residues), see Phobius details amino acids 208 to 232 (25 residues), see Phobius details amino acids 268 to 286 (19 residues), see Phobius details amino acids 297 to 316 (20 residues), see Phobius details amino acids 334 to 351 (18 residues), see Phobius details amino acids 360 to 387 (28 residues), see Phobius details amino acids 425 to 445 (21 residues), see Phobius details TIGR00783: citrate carrier protein, CCS family" amino acids 18 to 441 (424 residues), 585.3 bits, see alignment E=3.7e-180 PF03390: 2HCT" amino acids 23 to 440 (418 residues), 502.8 bits, see alignment E=3.1e-155

Best Hits

Swiss-Prot: 94% identical to CITN_KLEPN: Citrate-sodium symporter (citS) from Klebsiella pneumoniae

KEGG orthology group: None (inferred from 95% identity to kpn:KPN_00033)

MetaCyc: 69% identical to citrate:Na+ symporter (Vibrio cholerae O1 biovar El Tor str. N16961)
TRANS-RXN-501

Predicted SEED Role

"Na(+)Citrate OH(-) antiporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYE0 at UniProt or InterPro

Protein Sequence (446 amino acids)

>BWI76_RS04430 citrate carrier protein (Klebsiella michiganensis M5al)
MTNMSQAPSAEKKGVSDILGFKIFGMPLPLYAFALITLLLSHFYNALPTDIVGGFAIMFI
IGAVFGEIGKRLPIFNKYIGGAPVMIFLVAAYFVYAGIFTQKEIEAITNVMDKSNFLNLF
IAVLITGAILSVNRKLLLKSLLGYIPTILMGILGASIFGILIGLCFGISVDRIMMLYVLP
IMGGGNGAGAVPLSEIYHSVTGRSREEYYSTAIAILTIANIFAIVFAALLDIIGKKHTWL
SGEGELVRKASFKVEDDEKAGQITHRETAVGLVLATTCFLLAYVIAKKILPSIGGVSIHY
FAWMVLIVAALNASGLCSPEIKAGAKRLSDFFSKQLLWVLMVGVGVCYTDLQEIIDAITF
ANVVIAAVIVVGAVVGAAVGGWLIGFFPIESAITAGLCMANRGGSGDLEVLSACNRMNLI
SYAQISSRLGGGIVLVIASIVFGMMM