Protein Info for BWI76_RS04410 in Klebsiella michiganensis M5al

Annotation: signal transduction histidine kinase regulating citrate/malate metabolism

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 174 to 196 (23 residues), see Phobius details PF17203: sCache_3_2" amino acids 43 to 172 (130 residues), 77 bits, see alignment E=2.4e-25 PF14501: HATPase_c_5" amino acids 429 to 522 (94 residues), 40.3 bits, see alignment E=4e-14 PF02518: HATPase_c" amino acids 433 to 536 (104 residues), 65.6 bits, see alignment E=8.1e-22

Best Hits

Swiss-Prot: 91% identical to CITA_KLEPN: Sensor histidine kinase CitA (citA) from Klebsiella pneumoniae

KEGG orthology group: K07700, two-component system, CitB family, cit operon sensor histidine kinase CitA [EC: 2.7.13.3] (inferred from 91% identity to kpn:KPN_00029)

Predicted SEED Role

"Sensor kinase CitA, DpiB (EC 2.7.3.-)" in subsystem Orphan regulatory proteins (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYH2 at UniProt or InterPro

Protein Sequence (542 amino acids)

>BWI76_RS04410 signal transduction histidine kinase regulating citrate/malate metabolism (Klebsiella michiganensis M5al)
MFARKITHWFARRSFQNRIFLLILFTSTIVMLAMSWYLTNITEERLHYQVGQRALIQAMQ
ISAMPELVDAVEKHDLPRIKALIDPMRSFSDATYITVGDAKGQRLYHVNPEEIGKYMEGG
DSDEALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTIEQLENWLSLQIGS
LMIPMAIMLLLLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHY
RITAINQTARRLLNLSQPETILIGKSINSVISQEAFFYDAPQTNKKDEIVTFNQIKVIAS
RMAVTLNSEPQGWVISFRSKDDINTLSLQLSQVQQYADNLRAVQHEHRNLISTIAGLLFL
KRYDNALDLIQQQSESHQKVLDFIARNFKVNHLAGLLIGKYYRAKELGIELVFDPACHVE
TLPSSLSHNEWISIVGNLLDNAYNATLRQPTGSRQIECLINSEGDEAIVEIADQGCGIDE
SLRDRIFERGVTSSDSGDHGIGLWLVRSYVEQAGGNIVVDNNIPFGTIFTLYIPLTRDEH
HG