Protein Info for BWI76_RS04360 in Klebsiella michiganensis M5al

Annotation: ribonucleoside hydrolase RihC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 5 to 293 (289 residues), 312.8 bits, E=1.6e-97

Best Hits

Swiss-Prot: 94% identical to RIHC_KLEP7: Non-specific ribonucleoside hydrolase RihC (rihC) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K12700, non-specific riboncleoside hydrolase [EC: 3.2.-.-] (inferred from 93% identity to kva:Kvar_4370)

MetaCyc: 84% identical to ribonucleoside hydrolase RihC (Escherichia coli K-12 substr. MG1655)
Adenosine nucleosidase. [EC: 3.2.2.7]; Uridine nucleosidase. [EC: 3.2.2.7, 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.7, 3.2.2.3]; Inosine nucleosidase. [EC: 3.2.2.7, 3.2.2.3, 3.2.2.2]; Purine nucleosidase. [EC: 3.2.2.7, 3.2.2.3, 3.2.2.2, 3.2.2.1]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• superpathway of purines degradation in plants (15/18 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Glycosaminoglycan degradation
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.-.-, 3.2.2.1

Use Curated BLAST to search for 3.2.-.- or 3.2.2.1 or 3.2.2.2 or 3.2.2.3 or 3.2.2.7

Sequence Analysis Tools

See A0A285AYG2 at UniProt or InterPro

Protein Sequence (304 amino acids)

>BWI76_RS04360 ribonucleoside hydrolase RihC (Klebsiella michiganensis M5al)
MRLPIILDTDPGIDDAAAIAAALFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAGV
PLAQGASMPLVRPLRDAASVHGESGMEGYDFVEHHRQPLAKPAFQAIRDALMHAPEPMTL
VAIGPLTNIALLLTQYPECRFNIRRLVIMGGSAGRGNFTPNAEFNIAIDPEAAAKVFSSG
LEIVMCGLDVTNQAMLAPAYLATLPTLNQTGKMLHALFSHYRSGSMSSGLRMHDLCAIAW
LVRPQLFTLAPCFVAVETQGIWTSGTTVVDIEGKLGQPANAQVALGLDVEGFQRWAAEVI
ALAP